Project description:The main goal of this work was to determine the connection between MEF2C expression and its role in miRNA biogenesis in the differentiation of human skeletal muscle cells.In summary, our results demonstrated a significant role for MEF2C myogenic factor in sculpting the microtranscriptome during the differentiation of skeletal muscle cells.
Project description:The main goal of this work was to determine the connection between MEF2C expression and its role in miRNA biogenesis in the differentiation of human skeletal muscle cells.In summary, our results demonstrated a significant role for MEF2C myogenic factor in sculpting the microtranscriptome during the differentiation of skeletal muscle cells.
Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of growing myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using NIA15K mouse chips.
Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of growing myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using NIA15K mouse chips. Control and knock down cells were grown in proliferating conditions
Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of 28hr differentiated myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using affymetrix mouse chips.
Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of quiescent myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using affymetrix mouse chips.
Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of 28hr differentiated myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using affymetrix mouse chips. Control and knock down cells were grown in differentiating conditions for 28hrs and total RNA was used to perform microarray analysis
Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of quiescent myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using affymetrix mouse chips. Control and knock down cells were synchronized at G0 by suspension culture and total RNA was used to perform microarray analysis
Project description:Knockdown of MLL5 led to deregulation of S phase. To understand the molecular basis for this phenotype, we performed microarray analysis of S phase synchronized myoblasts. Genes differentially regulated by MLL5 knock down were revealed by microarray analysis using NIA15K mouse chips.