Schizosaccharomyces pombe
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA525996 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Suppressor screen for separase-securin and cohesin ts mutants in fission yeast
| PRJNA450289 | ENA
Project description:Interaction between TBP and condensin drives the organization and faithful segregation of mitotic chromosomes
| PRJNA257673 | ENA
Project description:Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast
| PRJNA369799 | ENA
Project description:Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast
| PRJNA369797 | ENA
Project description:Analysis of cohesin-dependent gene regulation in fission yeast
| PRJNA129961 | ENA
Project description:Cohesin connects CTCF binding sites and other genomic loci in cis to form chromatin loops, and replicated DNA molecules in trans to mediate sister chromatid cohesion. Whether cohesin uses distinct or related mechanisms to perform these functions is unknown. Here we describe a cohesin hinge mutant, which can extrude DNA into loops but is unable to mediate cohesion. Our results suggest that the latter defect arises during cohesion establishment. The observation that cohesin’s cohesion and loop extrusion activities can be separated indicates that cohesin uses distinct mechanisms to perform these two functions. Unexpectedly, the same hinge mutant can also not be stopped by CTCF boundaries as well as wildtype cohesin. This suggests that cohesion establishment and cohesin’s interaction with CTCF boundaries depend on related mechanisms and raises the possibility that both require transient hinge opening to entrap DNA inside the cohesin ring.
2023-10-25 | GSE235770 | GEO
Project description:Cohesin connects CTCF binding sites and other genomic loci in cis to form chromatin loops, and replicated DNA molecules in trans to mediate sister chromatid cohesion. Whether cohesin uses distinct or related mechanisms to perform these functions is unknown. Here we describe a cohesin hinge mutant, which can extrude DNA into loops but is unable to mediate cohesion. Our results suggest that the latter defect arises during cohesion establishment. The observation that cohesin’s cohesion and loop extrusion activities can be separated indicates that cohesin uses distinct mechanisms to perform these two functions. Unexpectedly, the same hinge mutant can also not be stopped by CTCF boundaries as well as wildtype cohesin. This suggests that cohesion establishment and cohesin’s interaction with CTCF boundaries depend on related mechanisms and raises the possibility that both require transient hinge opening to entrap DNA inside the cohesin ring.
2023-10-25 | GSE235771 | GEO
Project description:Cell-cycle regulation of cohesin stability along fission yeast chromosomes
| PRJNA101527 | ENA
Project description:genome re-sequencing of suppressor mutants of ptr1-1
| PRJDB1937 | ENA
Project description:Polyadenylation of pre-mRNAs, a critical step in eukaryotic gene expression, is mediated by cis elements, collectively called the polyadenylation signal. Genome-wide analysis of such polyadenylation signals was missing in fission yeast, which is an important model organism. We demonstrate that the canonical AATAAA motif is the most frequent and functional polyadenylation signal in Schizosaccharomyces pombe. Using analysis of RNA-Seq datasets from cells grown under various physiological conditions, we identify 3' UTRs for nearly 90% of the yeast genes. Heterogeneity of cleavage sites is common, as well as alternative polyadenylation within and between conditions. We validated the computationally identified sequence elements likely to promote polyadenylation, by functional assays including qRT-PCR and 3'RACE analysis. The biological importance of the AATAAA motif is underlined by functional analysis of genes containing it. Furthermore, it has been shown that convergent genes require trans elements, like cohesin for efficient transcription termination. Here we show that convergent genes lacking cohesin are generally (on chromosome 2) associated with longer overlapping mRNA transcripts. Our bioinformatic and experimental genome-wide results are summarized and can be accessed and customized in a user-friendly database Pomb(A).
2013-09-01 | E-MTAB-1824 | biostudies-arrayexpress