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Transcriptomes of maize seedlings under water and salinity treatment

ABSTRACT: Transcriptomes of maize seedlings under water and salinity treatment

PROVIDER: PRJNA527733 | ENA |

REPOSITORIES: ENA

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Transcriptomes of maize seedlings under water and salinity treatment

Project description:Purpose: We aim to reveal maize transcriptomic changes with water and salinity treatment. Methods: RNA-seq were used to reveal transcriptome of maize biological replicates with water and salinity treatment. Results: Differentially expressed transcripts were identified by the comparison of biological replicates with water and salinity treatment. Conclusions: We identified differentially expressed genes in respone to salinity treatment in maize.

2022-03-15 | GSE128432 | GEO

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Project description:Maize rough dwarf disease (MRDD) is a severe disease that has been occurring frequently in southern China and many other Asian countries. MRDD is caused by the infection of Rice black streaked dwarf virus (RBSDV) and leads to significant economic losses in maize production. To well understand the destructive effects of RBSDV infection on maize growth, comparative proteomic analysis of maize seedlings under RBSDV infection was performed using an integrated approach involving LC-MS/MS and TMT labeling. Our study identified 7615 maize proteins, of which 6319 proteins were quantified. A total of 116 differentially expressed proteins (DEPs) were identified, including 35 up- and 81 down-regulated proteins under RBSDV infection. Enrichment analysis showed that the DEPs were most strongly associated with Cyanoamino acid metabolism, protein processing in ER, and ribosome-related pathways. Two sulfur metabolism-related proteins were significantly reduced, indicating that sulfur may participate in the resistance against RBSDV infection. Furthermore, 15 DEPs involved in six metabolic pathways were identified in maize under RBSDV infection. Our data revealed that the responses of maize to RBSDV infection were controlled by various metabolic pathways.

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Genome-wide expression of transcriptomes under waterlogging stress condition in subtropical maize

Project description:Genome-wide transcriptome analysis was performed to understand the expression pattern of transcriptomes in tolerant and susceptible subtropical maize genotypes under waterlogging stress condition. Waterlogging stress causes yield reduction in maize (Zea mays). It is important to dissect the genetic circuits that underlie the plant responses to waterlogging. So, the experiment was designed with the following objectives: to understand the expression pattern of transcriptomes in the tolerant and the susceptible genotypes under waterlogging stress; to identify DEGs functioning in important pathways underlying adaptive traits; to co-map bin locations of the transcriptomes with already known QTLs for waterlogging and find synteny with other species; and to generate gene co-expression networks to study cohorts of genes expressed together in modules and functional cluster, while comparing the two genotypes.

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