Transcriptome of mature erythrocytes
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ABSTRACT:
PROVIDER: PRJNA528391 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Purpose: This study identifies the top transcripts expressed in mature erythrocytes and the top transcripts expressed in the polysome fractions of mature erythrocyte lysate Methods: RNA from total erythrocyte lysate and polysome fractions was isolated and purified using GeneJet RNA purification column (Thermo Fisher Scientific). The RNA library was prepared using NEBNext ULTRA II RNA library preparation kit for Illumina. The libraries were sequenced using Illumina Seq. Results: The transcriptome analysis revealed the abundance of mRNAs encoding globins genes, long non-coding RNAs, and micro RNAs. Deciphering the role and functions of these non-coding RNAs in these specialised cells could enable us to understand their biology in greater detail
2019-03-22 | GSE128648 | GEO
Project description:It is generally believed that human mature erythrocytes do not possess functional ribosomes, and therefore cannot synthesize proteins. However, this dogma is not consistent with the long life of mature erythrocytes in the circulatory system. The mass-spectrometry analysis was done on highly pure preparation of human mature erythrocytes to identify the proteome. Results of the analysis show that there is no contamination from other cell types. We also demonstrate translation by polysome profiling, metabolic labelling and RiboPuromycylation. RNA-seq and quantitative RT-PCR assays revealed that HBA (alpha globin) and HBB (beta globin) transcripts are selectively translated. RNA-seq and translatome analyses revealed the presence of all necessary translation factors and aminoacyl tRNA synthetases.
2023-03-10 | PXD021774 | Pride
Project description:We employed high-throughput sequencing of both short (~18-24nt) and long (>200nt) RNAs in human erythrocytes. We obtained blood from five healthy individuals for the short (small) RNA-seq library preparations and blood from three individuals for the long RNA-seq library preparations. We identified an abundant, diverse population of RNAs. Both polyadenylated and nonpolyadenlated long RNAs were identified. Additionally, known and novel microRNAs were identified in the short RNA dataset using the probabalistic modeling algorithm miRDeep. These RNAs lend insight into erythrocyte biology and provide utility as potential biomarkers. To determine both shared and unique aspects of the erythrocyte long RNA transcriptome, we compared this transcriptome with that of the PBMC and CD34+ erythroid progenitor transcriptomes. Sequencing of RNAs from mature erythrocytes of healthy individuals
2015-11-19 | E-GEOD-63703 | biostudies-arrayexpress
Project description:Human erythrocytes lack a nucleus and are considered to be incapable of protein synthesis. Nevertheless these anucleated cells regulate a number of gene expressions as well as posttranscriptional and postranslational modifications of proteins. We evaluated which genes are transcribed in mature erythrocytes. Keywords: expression profile
2006-02-01 | GSE3674 | GEO
Project description:Human erythrocytes lack a nucleus and are considered to be incapable of protein synthesis. Nevertheless these anucleated cells regulate a number of gene expressions as well as posttranscriptional and postranslational modifications of proteins. We evaluated which genes are transcribed in mature erythrocytes. Experiment Overall Design: Seven independent samples from healthy human donors were analyzed. Genes that had a detection call "present" in all seven array experiments were selected for further analysis.
2008-06-12 | E-GEOD-3674 | biostudies-arrayexpress
Project description:We employed high-throughput sequencing of both short (~18-24nt) and long (>200nt) RNAs in human erythrocytes. We obtained blood from five healthy individuals for the short (small) RNA-seq library preparations and blood from three individuals for the long RNA-seq library preparations. We identified an abundant, diverse population of RNAs. Both polyadenylated and nonpolyadenlated long RNAs were identified. Additionally, known and novel microRNAs were identified in the short RNA dataset using the probabalistic modeling algorithm miRDeep. These RNAs lend insight into erythrocyte biology and provide utility as potential biomarkers. To determine both shared and unique aspects of the erythrocyte long RNA transcriptome, we compared this transcriptome with that of the PBMC and CD34+ erythroid progenitor transcriptomes.
2015-11-19 | GSE63703 | GEO
Project description:Understanding gene expression changes over the lifespan of cells is of fundamental interest and gives important insights into processes related to maturation and ageing. This study was undertaken to understand the global transcriptome changes associated with ageing in fish erythrocytes. Fish erythrocytes retain their nuclei throughout their lifetime and they are transcriptionally and translationally active. However, they lose important functions during their lifespan in the circulation. We separated rainbow trout (Oncorhynchus mykiss) erythrocytes into young and old fractions using fixed angle-centrifugation and analyzed transcriptome changes using RNA sequencing (RNA-seq) technology and quantitative real-time PCR. We found 930 differentially expressed between young and old erythrocyte fractions; 889 of these showed higher transcript levels in young, while only 34 protein-coding genes had higher transcript levels in old erythrocytes. In particular ion binding, signal transduction, membrane transport, and various enzyme classes are affected in old erythrocytes. The transcripts with higher levels in old erythrocytes were associated with 7 different GO terms within biological processes and 9 within molecular functions and cellular components respectively. Our study furthermore found several highly abundant transcripts as well as a number of differentially expressed genes for which the protein products are currently not known revealing the gaps of knowledge in most non-mammalian vertebrates. Our data provide the first insight into changes involved in ageing on the transcriptional level and thus opens new perspectives for the study of maturation processes in fish erythrocytes.
2018-02-13 | GSE106570 | GEO
Project description:Piscine reovirus (PRV) is a causative agent of heart and skeletal muscle inflammation in Atlantic salmon, which is propagated in red blood cells (RBC). Here, transcriptome analyses of PRV infected erythrocytes showed strong and complex innate antiviral responses.
2021-08-30 | GSE183005 | GEO
Project description:Purpose: GRO-seq is a robust method to examine the nascent RNA transcriptome in the genome level Methods: The GRO-seq measures the trancription of nascent RNAs in the genome. From human CD34+ erythrocytes treated with veichle or T4 we conducted GRO-seq to examine the transcripome.
2017-09-06 | GSE102819 | GEO
Project description:Human stem cell derived reticulocytes were compared with mature erythrocytes by metabolomics analysis.
2016-05-11 | ST000403 | MetabolomicsWorkbench