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Translation in human mature erythrocytes

ABSTRACT: It is generally believed that human mature erythrocytes do not possess functional ribosomes, and therefore cannot synthesize proteins. However, this dogma is not consistent with the long life of mature erythrocytes in the circulatory system. The mass-spectrometry analysis was done on highly pure preparation of human mature erythrocytes to identify the proteome. Results of the analysis show that there is no contamination from other cell types. We also demonstrate translation by polysome profiling, metabolic labelling and RiboPuromycylation. RNA-seq and quantitative RT-PCR assays revealed that HBA (alpha globin) and HBB (beta globin) transcripts are selectively translated. RNA-seq and translatome analyses revealed the presence of all necessary translation factors and aminoacyl tRNA synthetases.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ling Li

LAB HEAD: Sandeep M Eswarappa

PROVIDER: PXD021774 | Pride | 2023-03-10

REPOSITORIES: Pride

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Evidence for low-level translation in human erythrocytes.

Kumar Sangeetha Devi SD Kar Debaleena D Akhtar Md Noor MN Willard Belinda B Roy Debadrita D Hussain Tanweer T Rajyaguru Purusharth I PI Eswarappa Sandeep M SM

Molecular biology of the cell 20220817 12

It is generally believed that human mature erythrocytes do not possess functional ribosomes and therefore cannot synthesize proteins. However, the absence of translation is not consistent with the long lifespan of mature erythrocytes. They stay viable and functional for about 115 d in the circulatory system. Here, using a highly pure preparation of human mature erythrocytes, we demonstrate the presence of translation by polysome profiling, [<sup>35</sup>S]methionine labeling, and RiboPuromycylat ...[more]

PMID: 35976696

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