Ontology highlight
ABSTRACT:
PROVIDER: PRJNA528459 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiles of [ESI+]
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Project description:We report the transcriptional profiles of the [ESI+] prion, as well as snt1∆, set3∆, and hos2∆ diploid cells.
2020-02-29 | GSE128672 | GEO
Project description:Transcription Profiles of [ESI+] and Set3C loss of function
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Project description:We profile the transcriptional landscapes associated with acquisition of [ESI+], a prion scaffolded by Snt1, a core component of the Set3C histone deacetylase. We find that acquisition of [ESI+] leads to expression of otherwise silent, heterochromatic loci, proposing a new molecular means by which silent transcriptional states might be reversed.
2020-02-29 | GSE117624 | GEO
Project description:Eriocheir sinensis isolate:Esi-2015 Genome sequencing
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Project description:Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a powerful method for profiling histone modifications and transcription factors binding throughout the genome. However, its application in economically important plant organs (EIPOs) such as seeds, fruits, tubers and flowers is challenging due to their sturdy cell walls and complex constituents. Here, we present advanced ChIP (aChIP), an optimized ChIP-seq strategy that efficiently isolates chromatin from plant tissues while simultaneously removing plant cell walls and cellular constituents. aChIP enables precise profiling of histone modifications in all tested EIPOs as well as transcription factors and chromatin-modifying enzymes. Notably, it significantly enhances ChIP efficiency and uncovers numerous novel modified sites compared to previous methods in vegetativetissues. Remarkably, aChIP unveils the first histone modification landscape of dry rapeseed seeds, illuminating the intricate roles of histone marks in EIPOs. Together, aChIP is a potent, efficient, and sensitive approach for comprehensive chromatin protein profiling across virtually all plant tissues, advancing plant epigenomics and functional genomics research, particularly within EIPOs.
2024-04-28 | GSE243804 | GEO
Project description:Embryonic stem (ES) cells are used in cell therapy and tissue engineering due to their ability to produce different cells types. However, studies of ES cells that are derived from fertilized embryos have raised concerns about the limitations imposed by ethical and political considerations. Therefore, many studies of ES cells use the ES cells that are derived from unfertilized oocytes and adult tissue. Although parthenogenetic embryonic stem (ESP) cells also avoided ethical and political dilemmas and can be used in cell-based therapy, the ESP cells exhibit growth retardation problems. Therefore, to investigate the potential for muscle growth from genetically modified ESP cells, we established four ES cell types, including normal embryonic stem (ESN) cells, ESP cells, ESP cells that overexpress the Igf2 gene (ESI) and ESP cells with down-regulated H19 gene expression (ESH). Using these cells, we examined the expression profiles of genes that were related to imprinting and muscle using microarrays. Total RNA obtained from isolated genetically modified parthenogenetic mouse embryonic stem cells compared to parthenogenetic mouse embryonic stem cells. 2 Biological Replication.
2011-07-29 | E-GEOD-31031 | biostudies-arrayexpress