Genome-wide DNA methylation level analysis in plants overexpressing METS1
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PROVIDER: PRJNA528822 | ENA |
REPOSITORIES: ENA
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Project description:We report here with this study the genome-wide DNA methylation level analysis at single-nucleotide resolution by Next Gen bisulfite-sequencing using the Methyl-MaxiSeq platform from Zymo Research Services (Irvine, CA) in plants overexpressing the gene 5-Methyltetrahydropteroyltriglutamate Homocysteine Methyltransferase1 (METS1) comparatively to Col-0 plants both basal conditions and following Pseudomonas syringae DC3000 infection.
2019-05-14 | GSE128768 | GEO
Project description:rs07-02_npc48 - analysis of npc48 overexpressing plants - Identification of potential targets of the non-protein codding RNA npc48. - We have lines overexpressing npc48 that shhowed a leaf morphological phenotype (serrated leaves). The proportion of lines havin gthe phenotype is around 20% of total transformants. We wanted to compare the transcriiptome of npc48-overexpressing lines with and without phenotype together with a transformation control for an additional control. Keywords: gene knock in (transgenic)
2008-04-12 | GSE10679 | GEO
Project description:Transcription profiling of tomato plants overexpressing SlMYB14
| PRJNA597746 | ENA
Project description:adt10-01_wip2 - wip overexpressing plants - Identification of genes induced or repressed by WIP genes - The objective is to identify genes responsible for the phenotypes observed in WIP overexpressing lines. WIP transcription factors are involved in reproductive and vegetative development by acting on the remodeling of various tissues. The WIP related processes have a major impact, in particular on reproduction (sex determination, fertilization, development of the seed). The way of action of these transcription factors is probably conserved, their effect depending only on their specificity of expression. Two WIP genes, NTT and TT1, was overexpressed in Arabidopsis thaliana. The objective is to compare the common deregulated genes (and in particular repressed) in the transcriptome of these two overexpressor lines, to highlight their mode of action and the way they act on the tissue in which they are expressed. Keywords: gene knock in (transgenic),normal vs transgenic comparaison
2011-09-01 | GSE24019 | GEO
Project description:rs07-02_npc48 - analysis of npc48 overexpressing plants - Identification of potential targets of the non-protein codding RNA npc48. - We have lines overexpressing npc48 that shhowed a leaf morphological phenotype (serrated leaves). The proportion of lines havin gthe phenotype is around 20% of total transformants. We wanted to compare the transcriiptome of npc48-overexpressing lines with and without phenotype together with a transformation control for an additional control. 4 dye-swap - CATMA arrays
2008-04-12 | E-GEOD-10679 | biostudies-arrayexpress
Project description:T2 progenies of two transgenic lines overexpressing ERF transcription factor WIN1 were grown on soil in parallel under identical conditions. mRNA was extracted from pooled leaves from multiple plants of each line for the microarray experiement. Plants derived from two lines were used in this experiment: line 13, a "medium" overexpressor, and line 22, a strong overexpressor. Control mRNA was obtained from pooled control plants transformed using an empty binary vector. All plants were first selected on medium containing kanamycin before being transferred to soil. Keywords = wax epidermis transcription factor Arabidopsis Keywords: parallel sample
2004-02-28 | GSE1071 | GEO
Project description:DNA methylation is an important mechanism of plant epigenomes, involved in regulating gene expression under environment stress. Nitrogen (N) resource is a significant limiting factor for plant growth and productivity in both natural and agricultural systems. To cope with the flexible environmental N changes, transgenic technolgies have been applied to enhance the N use efficiency (NUE) in crops. Here, we use Whole Genome Bisulfite Sequencing (WGBS) and Methylated DNA Immunoprecipitation Sequencing (MEDIP-seq) to study genome-wide methylation across transgenic plants with higher NUE, transgenic controls and their wild types.
2020-01-31 | GSE94319 | GEO
Project description:Transcription profiling of rice plants overexpressing OsGH3.1
2008-12-21 | E-MEXP-1773 | biostudies-arrayexpress
Project description:DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for mammalian development and maintenance of DNA methylation following DNA replication in cells. The DNA methylation process generates S-adenosyl-L-homocysteine, a strong inhibitor of DNMT1. Here we report that S-adenosylhomocysteine hydrolase (SAHH/AHCY), the only mammalian enzyme capable of hydrolyzing S-adenosyl-L-homocysteine binds to DNMT1 during DNA replication. SAHH activates DNMT1 in vitro and its overexpression in mammalian cells leads to hypermethylation of the genome, whereas its inhibition by adenosine periodate resulted in hypomethylation of the genome. Hypermethylation was consistent in both gene bodies and repetitive DNA elements leading to both down- and up-regulation of genes. Similarly, hypomethylation led to both up- and down-regulation of genes suggesting methylated regions influence gene expression either positively or negatively. Cells overexpressing SAHH specifically up-regulated metabolic pathway genes and down-regulated PPAR and MAPK signaling pathways genes. Therefore, we suggest that alteration of SAHH level in the cell leads to aberrant DNA methylation, altered metabolite levels and gene expression.
2018-06-06 | PXD006476 | Pride
Project description:adt10-01_wip2 - wip overexpressing plants - Identification of genes induced or repressed by WIP genes - The objective is to identify genes responsible for the phenotypes observed in WIP overexpressing lines. WIP transcription factors are involved in reproductive and vegetative development by acting on the remodeling of various tissues. The WIP related processes have a major impact, in particular on reproduction (sex determination, fertilization, development of the seed). The way of action of these transcription factors is probably conserved, their effect depending only on their specificity of expression. Two WIP genes, NTT and TT1, was overexpressed in Arabidopsis thaliana. The objective is to compare the common deregulated genes (and in particular repressed) in the transcriptome of these two overexpressor lines, to highlight their mode of action and the way they act on the tissue in which they are expressed. Keywords: gene knock in (transgenic),normal vs transgenic comparaison 6 dye-swap - CATMA arrays
2011-08-31 | E-GEOD-24019 | biostudies-arrayexpress