Project description:This research work investigates the expression of the genes involved in flavor compound production in two hybrids between Saccharomyces cerevisiae and S. kudriavzevii under low (12°C) and moderate fermentation temperatures (28°C).
Project description:Comparative genome-wide gene expression analysis between two industrial wine yeast hybrid strains belonging to the species S. cerevisiae x S. Kudriavzevii, in natural must fermentations.
Project description:Comparative genome-wide gene expression analysis between a wine yeast strain belonging to the species S. cerevisiae and the type strain from S. kudriavzevii IFO1802, a cryotolerant yeast, in natural must fermentations.
Project description:Analysis of genomic variation in several Saccharomyces cerevisiae x S. kudriavzevii natural hybrids S. cerevisiae and S. kudriavzevii yeasts have been described among wine strains. These strains exhibit enological properties of interest for the wine industry. A preliminary characterization of these hybrids showed a trend to reduce the S. kudriavzevii fraction of the hybrid genome. As a first approach to understand the mechanisms involved in the genome changes occurred after hybridization that may account for differences in their enological properties, we characterized the genomic constitution of several wine S. cerevisiae x S. kudriavzevii strains by using a combined approach based on the RFLP analysis of gene regions, comparative genome hybridizations with S. cerevisiae DNA arrays, ploidy analysis and gene dose determination by quantitative real-time PCR. Genomic DNA hybridizations of w27, w46, SPG1691 and 441 hybrids were compared against the laboratory strain FY1679. Hybridizations were performed by triplicate in order to obtain the final CGH ratio.
Project description:The aim of this experiment is to characterize the genome composition of the two hybrids IF6 and MR25. IF6 and MR25 are hybrids among S.cerevisiae and S. kudriavzevii. The hybridization of the genome extraction with the microarray was at 65C, under this condition only S. cerevisiae genes can hybridize. In this case we are only studying the genome composition of S. cerevisiae parental in the hybrids. The study of S. kudriavzevii parental genome was done by other methods.