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Single cell transcriptome of peritoneal cells

ABSTRACT: Single cell transcriptome of peritoneal cells

PROVIDER: PRJNA541920 | ENA |

REPOSITORIES: ENA

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Single cell transcriptome of peritoneal cells

Project description:To define the transcriptomic landscape of human mesothelial cells in response to PD, we analyzed single cell transcriptomes including cells dissociated from normal peritoneum (hernia surgery, n = 3), and peritoneal cells from effluent of short-term PD patients (PD less than 2 weeks, n = 6) and from long-term PD patients (PD more than 6 years, n = 4) ,we demonstrate that mesothelial cells develop hyperglycolysis, a metabolic alteration that is correlated with the development of peritoneal fibrosis.

2019-06-09 | GSE130888 | GEO

Single cell RNA-seq and BCR-seq of peritoneal B cells

Project description:B1 cells account for the majority of B cell population in the peritoneal cavity, and are essential for the innate immune responses and maintaining the homeostasis. The origin of the B1 cells and how to form the B1 cells pool in the postnatal life remain unknown. And the heterogeneity of B1 cells can largely affect the functions of B1 cells. Until now, nobody has performed the single cell RNA-seq of peritoneal B cells. In order to reveal the characteristics of peritoneal B cells, we have performed the scRNA-seq and scBCR-seq of the peritoneal B cells of mouse from different stages.

2022-05-21 | E-MTAB-10081 | biostudies-arrayexpress

Single cell RNA sequencing of total peritoneal cells from Beclinf/f-Lyz2cre mice

Project description:In Beclinf/f-Lyz2cre mice, we observed proinflammatory activation of tissue-resident peritoneal macrophages. These mice were resistent to lethal bacterial infection with Listeria. It is important to understand how the peritoneal immunity is affected by Beclin deletion in myeloid cells. To characterize the immune cells in the peritoneum, we performed single cell RNA sequencing of total peritoneal cells from Beclinf/f-Lyz2cre and littermate control Beclinf/f mice. The single cell RNA-seq analyses revealed profound changes in both the myeloid compartment and bystander cells.

2019-12-02 | GSE121521 | GEO

Single cell RNA-seq and BCR-seq of peritoneal B cells

Project description:Single cell RNA-seq and BCR-seq of peritoneal B cells

| PRJEB42996 | ENA

Peritoneal Dialysis Induces Alterations In The Transcriptome Of Peritoneal Cells Before Detectible Peritoneal Functional Changes

Project description:Long-term peritoneal dialysis (PD) is associated with functional and structural alterations of the peritoneal membrane. Inflammation may be the key moment and consequently fibrosis, the end result of chronic inflammatory reaction. The objective of the study was to identify genes involved in peritoneal alterations during PD by comparing transcriptome of peritoneal cells in short- and long-term PD patients. Peritoneal effluent of the long-dwell of stable PD patients was centrifuged to obtain peritoneal cells. Gene expression profiling of peritoneal cells using microarray between short- and long-term PD patients was compared. Based on microarray analysis 31 genes for RT-qPCR validation were chosen. A 4-hour peritoneal equilibration test (PET) was performed on the day after the long dwell. Transport parameters and proteins appearance rates were assessed. Genes involved in the immune system process, immune response, cell activation, leuko- and lymphocyte activation were found to be substantially up-regulated in the long-term group. RT-qPCR validation showed higher expression of CD24 (CD24 molecule), LY9 (lymphocyte antigen 9 ), TNFRSF4 (tumor necrosis factor receptor superfamily, member 4), CD79A (CD79a molecule, immunoglobulin-associated alpha), CCR7 (chemokine (C-C motif) receptor 7), CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) and IL2RA (interleukin 2 receptor, alpha) in long-term PD patients, CD24 having the best discrimination ability between short- and long-term treatment. A relationship between CD24 expression and genes for collagen and matrix formation was shown. Activation of CD24 provoked by pseudohypoxia due to extremely high glucose concentrations in dialysis solutions might play the key role in the development of peritoneal membrane alterations.

2019-01-24 | GSE125498 | GEO

Peritoneal Dialysis Induces Alterations In The Transcriptome Of Peritoneal Cells Before Detectible Peritoneal Functional Changes

Project description:Peritoneal Dialysis Induces Alterations In The Transcriptome Of Peritoneal Cells Before Detectible Peritoneal Functional Changes

| PRJNA516633 | ENA

Gene expression profile at single cell level of Peritoneal Cells in Patients with Gastric Cancer

Project description:We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of Peritoneal Cells in Patients with Gastric Cancer.

2024-04-17 | GSE228598 | GEO

Single cell RNAseq of male and female peritoneal macrophages

Project description:Single cell RNAseq of male and female peritoneal macrophages

| PRJNA587968 | ENA

Single cell RNA sequencing of total peritoneal cells from Beclinf/f-Lyz2cre mice

Project description:Single cell RNA sequencing of total peritoneal cells from Beclinf/f-Lyz2cre mice

| PRJNA497535 | ENA

Transcriptome of isolated mouse peritoneal endothelial cells

Project description:To characterize the metabolic profile of peritoneal endothelial cells (ECs) in response to peritoneal dialysis (PD), we performed RNA sequencing of peritoneal ECs isolated from mice treated with PD fluid for 6 weeks (n = 3) and from mice treated with saline for 6 weeks (n = 3). We demonstrated that peritoneal ECs had a hyperglycolytic metabolism in response to PD fluid treatment, which is associated with the development of microvascular alterations and peritoneal dysfunction.

2023-12-06 | GSE230008 | GEO

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