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Differential Gene expression of furin knockout (KO) DLD1, HCA7 and HT29 colorectal cancer cells

ABSTRACT: Differential Gene expression of furin knockout (KO) DLD1, HCA7 and HT29 colorectal cancer cells

PROVIDER: PRJNA542145 | ENA |

REPOSITORIES: ENA

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Differential Gene expression of furin knockout (KO) DLD1, HCA7 and HT29 colorectal cancer cells

Project description:We created furin KO cells in three colorectal cancer cell lines (DLD1, HCA7 and HT29) using CRISPR-Cas9 genome editing. And then we performed RNA-Seq analysis in furin KO colorectal cancer cells to identify potential effect of furin on gene expression patterns in DLD1, HCA7 and HT29 cells.

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Differential miRNA expression profiles of colorectal DLD1 and HT29 cell lines grown under 3D cell culture conditions

Project description:In the present study we investigated the microenvironment-dependent changes of miRNA expression in colorectal carcinoma DLD1 and HT29 cell lines. Next-generation sequencing technology was employed for the analysis of miRNA expression levels in cells cultivated under 3D multicellular spheroid and 2D culture conditions. The results of our study support the previous observations regarding the role of the tumor microenvironment in the regulation of gene expression. Furthermore, the profile of differentially expressed miRNAs, determined in the present study, could be applied for a further development of diagnostic and therapeutic applications in CRC.

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Single-cell RNA-seq analysis of WT and Furin KO TECs

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Furin deficiency efect on T cells

Project description:Analysis of T-cells lacking the proprotein convertase furin. Proprotein convertases promote the proteolytic maturation of proproteins. Furin is induced in activated T-cells. Results provide insight into the function of furin in T-cells. CD4+CD62L+CD44- naive, CD4+CD62L-CD44+ memory and CD4+CD25+FoxP3+ regulatory T cells were isolated from Fur flox/flox and CD4 cre Fur flox/flox mice. Naive T cells were activated via TCR. Total RNA was extracted from all cells and hybridized to Affymetrix microarrays.

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DLD1

Project description:DLD1 is an APC mut, KRAS mut, P53 mut CRC cell line. PROX1 transcription factor, target of Wnt pathway in CRC, is our protein of interest.DLD1 cells are PROX1 negative. We overexpressed through lentiviral expression PROX1 protein or the empty vector psd44, through selection of the cells in puromycin resistance. Afterwards we compared the transcriptional program of the DLD1-PROX1 and DLD1-Control cells growing in monolayer in vitro.

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Myeloid Cell Expressed Proprotein Convertase FURIN Attenuates Inflammation

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