Project description:The goal of the study was to analize expression of cell cycle related genes during postnatal development of Ts65Dn (Down syndrome mouse model) and control mice cerebellum. Keywords: Gene expression study in mouse model of disease
Project description:The goal of the study was to analize expression of cell cycle related genes during postnatal development of Ts65Dn (Down syndrome mouse model) and control mice cerebellum. Keywords: Gene expression study in mouse model of disease RNA from 3 controls and 3 Ts65Dn postnatal day 2 cerebellum were analized on separate arrays.
Project description:ATOH8 inhibits cell proliferation through transcriptional regulating cell cycle related genes, and ATOH8 and SMAD3 form a transcriptional complex to induce an anti-cell cycle signature and prevent cell cycle progression
Project description:linc00598 is a nuclear localized long noncoding RNA which transcribed in downstream of FoxO1. Previous studies showed that several nuclear retained lncRNAs function as transcriptional regulators. To find out the function of linc00598 as a transcriptional regulator, we performed microarray analysis using linc00598 knock down stable HEK293t cells. The results showed that positive cell cycle regulation related genes including cyclin D2 were regulated transcriptionally by linc00598. It was also observed that knock down of linc00598 induces G0/G1 cell cycle arrest and inhibit proliferation. Therefore linc00598 represents a novel cell cycle regulatory lncRNA and may be involved in promoting transformation of human cells.
Project description:Stem cell factor (SCF) mediated KIT receptor activation plays a pivotal role in mast cell growth, maturation and survival. However, the signaling events downstream from KIT are poorly understood. Mast cells express multiple regulatory subunits of class 1A PI3Kinase (PI3K) including p85α, p85β, p50α, and p55α. While it is known that PI3K plays an essential role in mast cells; the precise mechanism by which these regulatory subunits impact specific mast cell functions including growth, survival and cycling are not known. We show that loss of p85α impairs the growth, survival and cycling of mast cell progenitors (MCp). To delineate the molecular mechanism (s) by which p85α regulates mast cell growth, survival and cycling, we performed microarray analyses to compare the gene expression profile of MCps derived from WT and p85α-deficient mice in response to SCF stimulation. We identified 151 unique genes exhibiting altered expression in p85α-deficient cells in response to SCF stimulation compared to WT cells. Functional categorization based on DAVID bioinformatics tool and Ingenuity Pathway Analysis (IPA) software relates the altered genes due to lack of p85α to transcription, cell cycle, cell survival, cell adhesion, cell differentiation, and signal transduction. Our results suggest that p85α is involved in mast cell development through regulation of expression of growth, survival and cell cycle related genes. Two-condition experiment, wildtype vs. p85α-deficient mast cell progenitors stimulated with SCF. Biological replicates: 3 wildtype replicates, 3 p85α-deficient replicates.
Project description:Identification of cell cycle-related or cancer-related genes dysregulated by knockdown of MYC or lncRNAs in colorectal cancer-derived HCT116 or prostate cancer-derived PC3.