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Transcriptional Profiling of Clonal Cell Lines with Different Circadian Period

ABSTRACT: Transcriptional Profiling of Clonal Cell Lines with Different Circadian Period

PROVIDER: PRJNA548677 | ENA |

REPOSITORIES: ENA

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Clonal Cell Lines with Different Circadian Period

Project description:Clonal Cell Lines with Different Circadian Period

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Transcriptional Profiling of Clonal Cell Lines with Different Circadian Period

Project description:Composed of negative feedback loops, circadian oscillations are thought to be noise-resistant. Yet, individual cells in culture are remarkably heterogenous, oscillating independently and with different period lengths. To investigate the mechanisms underlying this heterogeneity, we generated and characterized hundreds of clonal cell lines from the same parent culture as well as subclones from clonal lines. By comparing clonal lines with different circadian periods, we identified a pool of candidate genes that determine periodicity.

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RRBS Profiling of Clonal Cell Lines with Different Circadian Period

Project description:Composed of negative feedback loops, circadian oscillations are thought to be noise-resistant. Yet, individual cells in culture are remarkably heterogenous, oscillating independently and with different period lengths. To assess whether differential methylation contributes to heritable heterogeneity of circadian periods, we used reduced representation bisulfite sequencing (RRBS) to explore DNA methylation profiles and their correlation with the transcriptome in the 10 clonal cell lines. To investigate the mechanisms underlying this heterogeneity, we generated and characterized hundreds of clonal cell lines from the same parent culture. By comparing clonal lines with different circadian periods, we identified a group of differentially methylated regions (DMRs).

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RRBS Profiling of Clonal Cell Lines with Different Circadian Period

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Clonal Cell Lines with Different Circadian Period

Project description:This SuperSeries is composed of the SubSeries listed below.

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Maternally expressed imprinted gene SDC modulates circadian period and hypocotyl length

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Nonsense mediated decay and a novel protein Period-2 regulate casein kinase I in an opposing manner to control circadian period in Neurospora crassa

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