Transcriptome analysis of HPV-induced warts and healthy skin in humans
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ABSTRACT:
PROVIDER: PRJNA562255 | ENA |
REPOSITORIES: ENA
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Project description:The main purpose of this study is to investigate the differences between the gene expression profiles of common warts and healthy skin in HPV-positive individuals by RNA sequencing on the Illumina HiSeq 2500. After obtaining shave biopsies of common warts and healthy skin from twelve Arab males, we were able to analyze the transcriptomes of 24 paired cases and controls.
2019-08-27 | GSE136347 | GEO
Project description:Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer in humans. HPV persistence in the epidermis and excessive wart or squamous is associated with cancer development. Various viral warts can be found on the skin infected with HPV, like condyloma acuminatum and bowenoid papulosis (BP). At present, it is believed that BP is a potential malignant squamous cell carcinoma in situ. In this study, we reported a very rare case of HPV-positive patient with concurrence of BP and cSCC. This patient progressed at a very fast rate and transferred to death quickly. To reveal the cell microenvironment changes from normal skin (NS) to BP and cSCC after HPV infection, we performed scRNA-seq on four pathological lesions skin (NS, BP, paracancerous squamous cell carcinoma (pc-cSCC) and cSCC) from this patient. Among immune cells, CD52+ macrophages were only detected in pc-cSCC and cSCC tissue, and two clusters of T cells, CD8+ Tex cells and CD4+ Tregs as well as matrix cells like MMP1+, MMP11+ fibroblasts were increased in pc-cSCC and cSCC tissue. Widely interactions were also found among specific T cells/macrophages and keratinocytes, which might be involve in the cSCC progress. Targeting these cells or their interactions may be beneficial for the diagnosis and treatment of cSCC.
2024-04-05 | GSE262947 | GEO
Project description: Not available
| S-EPMC7063766 | biostudies-literature
Project description:A research project is currently being undertaken looking at Human Papilloma Virus (HPV) vaccination in special risk groups. It aims to see if young women with a chronic illness respond well to the HPV vaccine or whether they may require additional doses to ensure protective immunity. The four valent HPV vaccine protects against HPV types 16 & 18, cervical cancer and HPV types 6 & 11, anogenital warts.
The six special risk groups include:
Paediatric Rheumatological Disease Inflammatory Bowel Disease Acute Lymphoblastic Leukaemia Solid Organ Transplant Recipients (kidney and liver) Chronic Renal Disease Bone Marrow Transplants This immunity is measured by antibody levels of the HPV types, which requires a single blood test one month after the final dose of HPV vaccine.
This is compared to healthy controls using antibody response to HPV vaccine. This will assess directly whether these special risk groups respond as well to the HPV vaccine.
| 2077042 | ecrin-mdr-crc
Project description:In humans, α-papillomavirus HPV-2 and γ-papillomavirus HPV-4 typically cause common warts. In rare patients, HPV-2 may cause striking “tree man” manifestations. We describe here a patient with the HPV-2-driven “tree man” phenotype, and two of his distant relatives with unusually severe HPV4-driven warts. Genetic, transcriptional, and histological analyses of the “tree man” lesions showed that they formed a multifocal benign epithelial tumor driven by a specific HPV-2 strain. Surprisingly, all three patients are homozygous for a mutation of an essential splice site in the CD28 gene, which encodes an activating surface receptor on T cells. They have no detectable CD28 on their T cells, which, with the exception of a small contingent of revertant memory CD4+ T cells, do not respond to CD28 stimulation. Their T cells express normal levels of other costimulatory molecules and CTLA-4, and respond to CD2 stimulation. T- and B-cell development, and responses to antigens are otherwise normal in these individuals. Finally, CD28-deficient mice are vulnerable to cutaneous infections with the μ-papillomavirus MmuPV1. This experiment of Nature shows that the control of HPV-2 and HPV-4 in human keratinocytes is dependent on the T-cell CD28 co-activation pathway, which seems to be otherwise largely redundant in host defense.
2021-07-31 | GSE139299 | GEO
Project description:Human papillomaviruses (HPV) preferentially infect keratinocytes of mucous membranes or skin and cause numerous benign and malignant lesions at different anatomical locations. We sequenced small RNA (sRNA) libraries of two HPV 16 immortalized cell lines and ten formalin fixed paraffin embedded (FFPE) tissue samples from HPV infected cervical epithelium by SOLiD 4 technology.
2020-06-30 | GSE62239 | GEO
Project description:Genome wide DNA methylation profiling of normal skin and common warts samples infected with the human papilloma virus (HPV). The Illumina Infinium MethylationEPIC BeadChip was used to obtain DNA methylation profiles across approximately 850,000 CpGs. DNA was extracted from 24 paired wart and normal skin samples.
2023-05-24 | GSE213888 | GEO
Project description:Hidradenitis suppurativa (HS) is an inflammatory skin disease with limited therapeutic options. CD4 T Cells have been described as more inflammatory than T cells in healthy skin. To better understand alterations within the T cell compartment, we profiled CD4 Teffector cells and regulatory T cells (Treg) from inflammatory HS skin lesions and healthy control skin via scRNASequencing.
2023-12-07 | GSE249622 | GEO