Project description:454-pyrosequencing technology was employed to investigate the microbial communities from anaerobic fluidized bed reactor treating laundry wastewater.
Project description:Genome-wide epigenetic changes such as histone modifications form a critical layer of gene regulations and have been implicated in a number of different disorders such as cancer and inflammation. Progress has made to decrease the input required for gold-standard genome-wide profiling tools like chromatin immunoprecipitation followed by next generation sequencing (i.e. ChIP-seq) to allow scarce primary tissues of specific type from patients and lab animals to be tested. However, there has been very little effort to rapidly increase the throughput of these low-input tools. In this report, we demonstrate LIFE-ChIP-seq (Low-Input Fluidized-bed Enabled Chromatin Immunoprecipitation combined with sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays in as little as 1 h with as few as 50 cells per assay. Our technology will enable testing of a large number of samples and replicates with low-abundance primary samples in the context of precision medicine.
2018-06-01 | GSE102932 | GEO
Project description:Robustness and microbial diversity of a Fluidized Bed Reactor (FBR) employed to treat diluted laundry wastewater without supplementary feeding
Project description:Purpose: To uncover the mechanism of 4-nonylphenol-induced impairment of sertoli cells, we used RNA sequencing technology to compare the small RNA expression differences between the control groups and the 4-nonylphenol treated groups. Sertoli cells small RNA profiles of the control groups and the 4-nonylphenol treated groups were generated by deep sequencing, in triplicate. The library quality was determined using a Bioanalyzer 2100 (Agilent). The Illumina BGISEQ platform was used for small RNA sequencing. The quality of RNA-seq reads was examined using FastQC.
