Project description:We examined gene expression profiling of native mussels that were sampled in early summer 2003 from sites of the Venice lagoon area known to be differently affected by chemical pollution: Sites 1 and 2 close to the industrial district of Marghera and Site 3 close to the Lido lagoon outlet. Site 4, a current mussel farm located offshore, has been chosen as source of reference targets for microarray hybridizations. We have limited the preliminary assessment to the digestive gland. Digestive gland total RNA of each Site was hybridized in competition with the offshore mussels (Site 4 - Reference) and the relative abundance of each gene was measured by directly comparing fluorescent signals for each probe. We carried out two separate hybridizations for each site of the Venice lagoon area.. Keywords = digestive gland Keywords = Venice lagoon Keywords = chemical pollution Keywords = native mussels Keywords = transcriptional profiling Keywords: ordered
Project description:The dataset comprises the most abundant and largest (by stem diameter) tree species in the Barro Colorado Island 50-ha forest dynamics plot in Panama, as well as all local species in 7 of the most species-rich genera in the plot: Eugenia (Myrtaceae), Inga (Moraceae), Miconia/Clidemia (Melastomataceae), Ocotea/Nectandra (Lauraceae), Piper (Piperaceae), Protium (Burseraceae), Psychotria/Palicourea (Rubiaceae). Leaf samples were extracted with 90:10 methanol:water pH 5 and analyzed using methods described in Sedio et al. 2017 Applications in Plant Sciences (doi:10.1002/aps3.1033).
Project description:DNA microarray analyses of Ruditapes philippinarum sampled in Venice lagoon areas subjected to different anthropogenic impact. A comparative analysis of gene expression was conducted between Manila clam from lowly-polluted Chioggia and Colmata area and polluted Marghera site.
Project description:A Ruditapes philippinarum microarray platform was developed to assess variations on transcritpomic response to copper exposures in Manila clam colelctted in Venice lagoon areas subjected to different anthropogenic impact