Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MRNA structure regulates protein expression through changes in functional half-life

ABSTRACT: mRNA structure regulates protein expression through changes in functional half-life

PROVIDER: PRJNA578697 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

mRNA structure regulates protein expression through changes in functional half-life

Project description:A SHAPE-MaP structure probing experiment was performed on 39 firefly luciferase mRNAs containing uracil, 1-methyl-psuedouracil, or 5-methoxy-uracil. For each mRNA, SHAPE-MaP includes a sample treated with 1M6 ('MOD'), a minus reagent ('NC'), and a denatured control ('DEN'). The 1M6 reagent preferenctially reacts with unpaired bases in RNA and subsequently induces mutations during the reverse transcription step of library preparation. After sequencing and alignment, the 'mutational profiles' of the 'p', 'm', and 'd' samples are used to calculate the SHAPE reactivity of each base.

2019-10-22 | GSE139176 | GEO

mRNA structure regulates protein expression through changes in functional half-life.

Project description: Not available

| S-EPMC6883848 | biostudies-literature

Cell cycle mRNA half-life profiling.

Project description:mRNA half-life profiling in the bacterium Caulobacter crescentus was performed in synchronized cells collected from different stages of the cell cycle including swarmer cells, stalk cells (45 min post synchrony), and predivisional cells (90 min post synchrony). For each cell population, transcription was disrupted by the antibiotic rifampicin, and RNA samples were collected at different time points to measure the mRNA half-lives.

2023-04-03 | GSE228593 | GEO

BR-body mutant mRNA half-life profiling.

Project description:BR-body mutant mRNA half-life profiling.

| PRJNA551694 | ENA

BR-body mutant mRNA half-life profiling.

Project description:BR-body mutant strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. JS38 (wild type) was compared to JS221 (lacking the intrinsically disordered CTD and unable to form BR-bodies), JS233 (unable to recruit degradosome components into BR-bodies), and JS299 (active site mutant of RNase E).

2019-06-29 | GSE133532 | GEO

WT, ∆rhlB and ∆rppH mRNA half-life profiling.

Project description:WT, ∆rhlB and ∆rppH strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. Wild type(WT) was compared to ∆rhlB (deleted RNA helicase RhlB gene) and ∆rppH (deleted RppH gene) strains

2023-04-03 | GSE228592 | GEO

NANOS2-binding regulates mRNA half-life and key spermatogonial stem cell self-renewal pathways

Project description:We have identified NANOS2 bound targets transcriptome wide by employing CRAC and RNA-seq in mouse SSCs. We have determined the average mRNA half-life in SSCs by SLAM-seq. By comparing CRAC and SLAM-seq datasets, we have demonstrated that NANOS2 binding reduces mRNA stability in SSCs.

2021-05-06 | GSE149835 | GEO

Retapamullin and Chloramphenicol treated mRNA half-life profiling.

Project description:mRNA half-life profiling in the bacterium Caulobacter crescentus was performed in cells that were inhibited in translation initiation (retapamullin) or elongation (chloramphenicol) by shutting of transcription with the antibiotic rifampicin, and following mRNA abundance at 1, 2, 4, 8, and 15 minutes post rifampicin. All RNA measurements were performed on cells grown to mid-log in M2G minimal growth medium. Two biological replicates time coursees were collected from independent starter cultures.

2023-04-03 | GSE228591 | GEO

WT and RNE∆IDR mRNA half-life profiling in Sinorhizobium meliloti.

Project description:WT and RNE∆IDR strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. Wild type(WT) was compared to RNE∆IDR strain.

2023-12-25 | GSE251703 | GEO

Database for mRNA Half-Life of 19,977 Genes Obtained by DNA Microarray Analysis of Pluripotent and Differentiating Mouse

Project description:Degradation of mRNA is one of the key processes that control the steady-state level of gene expression. However, the rate of mRNA decay for the majority of genes is not known. We successfully obtained the rate of mRNA decay for 19 977 non-redundant genes by microarray analysis of RNA samples obtained from mouse embryonic stem (ES) cells. Median estimated half-life was 7.1 h and only <100 genes, including Prdm1, Myc, Gadd45 g, Foxa2, Hes5 and Trib1, showed half-life less than 1 h. In general, mRNA species with short half-life were enriched among genes with regulatory functions (transcription factors), whereas mRNA species with long half-life were enriched among genes related to metabolism and structure (extracellular matrix, cytoskeleton). The stability of mRNAs correlated more significantly with the structural features of genes than the function of genes: mRNA stability showed the most significant positive correlation with the number of exon junctions per open reading frame length, and negative correlation with the presence of PUF-binding motifs and AU-rich elements in 30 -untranslated region (UTR) and CpG di-nucleotides in the 50 -UTR. The mRNA decay rates presented in this report are the largest data set for mammals and the first for ES cells. Keywords: growth condition design,reference design,replicate design,RNA stability design,time series design

2008-11-15 | GSE13609 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data