Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis seedlings that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs). Arabidopsis seedlings were treated with 2 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.
Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs). Arabidopsis mesophyll protoplasts were treated with 100 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.
Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis seedlings that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs).
Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs).
Project description:To investigate flg22-induced posttranslational modification of BSU1 family proteins, we performed metabolic Stable Isotope Labeling In Arabidopsis followed by Immuno-Precipitation and Mass Spectrometry (SILIA IP-MS) analysis, with the isotopes switched in replicate experiments.
Project description:To confirm the flg22-induced BSU1 phosphorylation site, we performed targeted quantification by PRM analysis with SILIA IP-MS sample. The isotopes switched in replicate experiments.