Project description:In our study, we found that CBX4 was critical to osteosarcoma metastasis.CBX4, also known as polycomb 2 (Pc2), belongs to the CBX protein family, including CBX2, 4, 6, 7, and 8, which has been shown to participate in the polycomb repressive complex 1 (PRC1) and characterized as a transcriptional repressor. To identify the downstream target genes of CBX4 in osteosarcoma, we conducted the RNA-seq analysis of CBX4 overexpression or knockdown in osteosarcoma cell line U2OS/MTX300.
Project description:CRISPR screen: U2OS or U2OS p53KO cells expressing Cas9 were transduced with a whole-genome library of CRISPR sgRNAs, then treated with either DMSO or etoposide. Differential sgRNA abundances were calculated for each condition and used to determine the effect of each single gene knockout on fitness and the drug-induced death rate. RNA-Seq: U2OS or U2OS p53KO cells were cultured with either DMSO or etoposide for 48 hours, and then U2OS cells were incubated in this conditioned media for 8 hours. RNA was collected and use to observe differential expression changes between conditions.
Project description:lncRNA is reported to regulate gene transcription in variety of ways. hTR is a lncRNA with 451 nt. It is classical role is serving as template for telomere lengthening. However, it involves in some other biology processes as a lncRNA. To screen for genes regulated by hTR, we performed RNAseq with hTR expressed U2OS cells, using pBabe-U2OS as control.
Project description:ORCA is an ORC associated protein that plays important roles in replication initiation as well as heterochromatin organization. We carried out ORCA ChIP-seq in U2OS cells synchronized at different stage of G1 phase to determine its genome wide localization. To understand the genomic features of ORCA binding regions, we also carried out Methylated DNA IP (MeDIP) followed by deep sequencing in U2OS cells to determine the genome wide localizatoin of methyl-CpG sites in U2OS cells and how ORCA bidning regions co-localize with this important repressive mark.
Project description:To better understand the mechanism by which CFZ impacts polyQ toxicity, we conducted a transcriptomic analysis in polyQ94-EGFP expressing U2OS (5μM, 8 days). Data from a transcriptomics experiment in polyQ94-U2OS cells treated with CFZ (5 μM, 8day) was compared to a DMSO-treated control.
Project description:Identification of the DNA binding landscape of the transcription factor regulatory factor X 7 (RFX7) in Nutlin-3a and DMSO control treated U2OS cells.
