Project description:The study provides a comparative of transcript levels in uninfected and CHIKV-infected Aedes aegypti derived Aag2 cells using RNA Seq
Project description:Loquacious-2 (Loqs2) is a recently identified paralog of Loquacious found in Aedes aegypti. Data presented here allowed the proteomic characterization of Loqs2 interactome in Aedes aegypti Aag2 cells. We carried out immunoprecipitation experiments where Loqs2 tagged protein was precipitated along with its partners. Immunoprecipitation of tagged eGFP protein was performed in the same conditions to verify the specificity of these associations. Experiments were performed in biological triplicates.
Project description:This analysis compare gene expression between 4 day old sugar fed female and male Aedes aegypti mosquitoes. Keywords: Aedes aegypti sex specific expression
Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti transcriptome 5 hours after blood feeding. Comparison of the transcriptome of Aedes aegypti females at two physiological conditions and one time point.
Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti transcriptome 5 hours after blood feeding.
Project description:PIWI-interacting (pi) RNAs are a class of small RNAs that have diverse functions in mosquitoes. In order to uncover novel components involved in biogenesis or function of the piRNA pathway in Aedes aegypti mosquitoes, we performed mass spectrometry analyses on immunoprecipitated PIWI proteins and their interactors.
Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti midguts transcriptome 4,8,18 hours after blood feeding BSA or DENV2 NS1.
Project description:The incomplete genome annotation of non-model organisms hampers molecular and proteomic studies. Proteomics informed by transcriptomics (PIT) is suited to non-model organisms because peptides are identified using transcriptomic, not genomic, data. Aedes aegypti is the mosquito vector for the (re-)emerging dengue, chikungunya, yellow fever and Zika viruses. An Ae. aegypti genome sequence is available, however experimental evidence for >90% of the Ae. aegypti proteome or the activity of transposable elements (TEs) that constitute 50% of the Ae. aegypti genome is lacking. We used PIT to characterise the proteome of the Aedes aegypti derived cell line Aag2. Hotspots of incomplete genome annotation were identified which are not explained by poor sequence and assembly quality. We developed criteria for the characterisation of proteomically active TEs and demonstrate that protein expression does not correlate with a TE’s genomic abundance. Finally, we identify Phasi Charoen-like virus as an unrecognised contaminant of Aag2 cells. We therefore present the first proteomic characterisation of mobile genetic elements, and provide proof-of-principle that PIT can evaluate a genome’s annotation to guide annotation efforts.