Project description:We report the application of single-cell-RNA sequencing technology for high-throughput profiling of primary mouse epidermal cells. We use a topical toll-like receptor 7 agonist, Imiquimod (IMQ) to activate the skin immune system. We find that the proportion of most of the cell types are conserved after IMQ treatment. We identify many interferon-sensitive genes that are induced after 6 hours of IMQ treatment. This study provides a deeper understanding of the cell type-specific anti-viral gene expression response to chemical modulators such as IMQ.
Project description:To clarify the role of DMD in macrophages in response to IMQ, we performed transcriptomic analysis by a Low Input Quick-Amp Labeling kit (Agilent Technologies, USA) from IMQ-induced THP1 macrophages with or without DMD treatment in vitro.
Project description:Analysis of the tag densities showed lower 5-hmC levels in both CpG islands and genebodies in Imiquimod (IMQ)-treated epidermal samples. We looked further into loci-specific changes of 5-hmC and found a total of 364 differentially hydroxymethylated regions. Within these regions, 321 genes showed significantly lower 5-hmC levels and 67 genes had significantly higher 5-hmC levels in the IMQ-treated epidermis compared to control.
Project description:We then performed gene expression profiling analysis using data obtained from RNA-seq of 9 skin tissues including 3 per group in vaseline-treated group, imq model group and bergapten-gel-treated imq model group.
Project description:The mechanisms underlying pruritus of imiquimod (IMQ)-induced psoriasis remain poorly understood. In this study, we investigated whether there are certain key signaling molecules downstream of the recently identified peptides mediating itch in the spinal cord. We investigated the changes in the trascriptome by performing RNAseq of cervical spinal cords in control and IMQ-induced psoriasis. Our study represents the detailed analysis of transcriptomes of spinal cord in chronic itch model with biologic replicates, generated by RNA-seq technology.
Project description:We performed MeRIP-seq on (1) two human epidermis tissue and HaCAT (human keratinocyte cell line) cells; (2) HaCAT cell lines with or without ALKBH5 knockdown by siRNA in duplicate experiments.
Project description:TREX2 is a keratinocyte specific 3â-deoxyribonuclease that participates in the maintenance of skin homeostasis upon damage. This transcriptome analysis identified multiple genes and pathways deregulated by TREX2 loss in the IMQ-induced psoriasis-like model in mouse skin. mRNA sequencing of 5 biological replicates of skin from wild-type mice treated with Imiquimod and 6 of Trex2 knockout mice treated with Imiquimod