Project description:Macrophages, which populate barrier tissues already in the embryo, are primary targets for CMV. Upon infection macrophages undergo a morphological, immunophenotypic and metabolic transformation process with features of stemness, altered migration, enhanced invasiveness and provision of the cell cycle machinery to viral proliferation.

2021-06-15 | GSE143446 | GEO

Cytomegalovirus subverts macrophage identity

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-06-15 | GSE159389 | GEO

Cytomegalovirus subverts macrophage identity [LysMcreZeb1flox_vs_LysMcreZeb1wt]

2021-06-15 | GSE167206 | GEO

Cytomegalovirus subverts macrophage identity [Alveolar macrophages]

2021-06-15 | GSE159388 | GEO

Bone marrow derived macrophage vs peritoneal macrophage

Project description:Bone marrow derived macrophage vs peritoneal macrophage

| PRJNA414129 | ENA

RNASeq profiles from two distinct F4/80+VCAM1+ sorted bone marrow populations are consistent with non-macrophage cells coated with macrophage fragments

Project description:The objective of this study was to characterise macrophage subsets in bone marrow (BM) isolated from Csf1r-EGFP mice. A concurrent imaging flow cytometry study conducted by our team unexpectedly revealed macrophage surface marker staining emanates from membrane-bound subcellular remnants associated with unrelated cells. Expression data from sorted BM “macrophage” populations was found to be consistent with macrophage fragments associated with non-macrophage cells. Granulocyte-specific genes were enriched within the CD11b+ “macrophage” (CD11b+F4/80+Ly6G-GFPloVCAM1+) populations, whereas CD11b- “macrophages” (CD11b-F4/80+GFP+VCAM1+) were consistent with a mixed cell population including both plasma cells and erythroblasts. This data demonstrates how fragmentation of hematopoietic tissue macrophages can result in misattribution of macrophage identity to non-macrophage populations, thereby undermining accuracy of macrophage ex vivo molecular profiles.

2021-11-05 | GSE187052 | GEO

PPARÎ³ controls alveolar macrophage identity [part2]

Project description:Tissue-resident macrophages comprise heterogeneous populations with unique functions and distinct gene expression signatures. While it has been established that they mostly originate from embryonic progenitors, the signals inducing a characteristic tissue-specific differentiation program remain unknown. Here we identify PPARÎ³ as the crucial transcription factor determining perinatal alveolar macrophage (AM) development and identity. Development of the fetal monocyte derived AM precursor was largely abrogated in CD11c-Cre/Ppargfl/fl mice. To reveal the underlying changes in gene expression, we performed microarray analysis of sorted WT and KO AM and pre-AM from 3 different timepoints. Part2: adult sorts & array AM FACS-sorted from lungs of adult PPARgfl/fl (WT) and CD11c-cre/PPARgfl/fl (KO) mice and subsequently processed for RNA extraction and hybridization on Affymetrix microarrays. 2 biological replicates per group, each composed of pooled cells from 2 individual mice

2014-09-28 | E-GEOD-60248 | biostudies-arrayexpress

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