Project description:RNAseq used to examine gene expression in thermal challenged redband rainbow trout RNAseq data obtained from libraries prepared from Gill RNA
Project description:The study was designed to investigate the impacts of hatchery spawning and rearing on steelhead trout (Oncorhynchus mykiss) versus the wild fish on a molecular level. Additionally, epigenetic differences between feeding practices that allow slow growth and fast growth hatchery trout were investigated. The sperm and RBC DNA both had a large number of DMRs when comparing the hatchery versus wild steelhead trout populations. Interestingly, the DMRs were cell type specific with negligible overlap. Slow growth compared to fast growth steelhead also had a larger number of DMRs in the RBC samples. Observations demonstrate a major epigenetic programming difference between the hatchery and wild fish populations, but negligible genetic differences. Therefore, hatchery conditions and growth rate can alter the epigenetic developmental programming of the steelhead trout, which may correlate to the phenotypic variations observed.
Project description:Responses of salmonid populations to ecosystem degradation often, if not typically, involve multiple stressors. The interaction of disease and contaminant exposure is of potential relevance to persistence of salmonid populations. The duration of PAH exposure and the life stage of exposure in the trout reflect a likely exposure scenario for some stocks of anadromous salmonids (sub-yearling ocean-type Chinook salmon) during emigration to the ocean. This current study focuses on the genes expressed in rainbow trout after exposure to both the high molecular weight PAH mixture and to the A. salmonicida pathogen.
