Project description:Gilthead sea bream fed plant-protein based diets with either fish oil or vegetable oil as the most iportant source of dietary lipids were experimentally exposed to the intestinal parasite Enteromyxum leei by water effluent. A specific gilthead sea bream oligo-microarray was used to determine the intestine transcriptomic response.
Project description:Gilthead sea bream fed plant-protein based diets with either fish oil or vegetable oil as the most iportant source of dietary lipids were experimentally exposed to the intestinal parasite Enteromyxum leei by water effluent. A specific gilthead sea bream oligo-microarray was used to determine the intestine transcriptomic response. 41 samples from six experimental groups (2 diets x 3 infective status) in a single-color hybridization
Project description:A gilthead sea bream (Sparus aurata) microarray platform was developed to identify brain gene expression profiles in response to environmental concentrations of human pharmaceuticals.
Project description:Explore the underlying mechanisms-of-action after short-term (24 h) waterborne exposure to low (0.5 μg/L) and high (50 μg/L) gold nanoparticles (AuNP) concentrations in gilthead sea bream.
Project description:A gilthead sea bream (Sparus aurata) microarray platform was developed to identify brain gene expression profiles in response to environmental concentrations of human pharmaceuticals. Comparative analysis of gene expression profiles was conducted among brain of gilthead seabream exposed to Acetaminophen (APAP; analgesic), Carbamazepine (CBZ; anti-epileptic) and Atenolol (AT; β-blocker). All groups of samples were also compared with brain of control individuals.
Project description:In this study, we analyzed both together the epithelial tissue and the secreted mucus response using a holistic interactome-based multi-omics approach. The effect of the gilthead sea bream (Sparus aurata) skin mucosa to a dietary inclusion of spray-dried porcine plasma (SDPP) was evaluated.
Project description:We report a comparison of tissue-specific (head kidney, intestine and liver) gene expression profiles from gilthead sea bream fed with control and Brewer's spent dry yeast (SDY) diets.The inclusion of SDY at 30% in the experimental diet (40% crude protein, 16% crude lipid) resulted in a reduction in FM (10%) and PP (31.4%) contents. 218 differentially expressed genes (DEGs) were identified among all tissues, out of which, 141 were up- and 77 down-regulated. The enrichment analysis of DEGs revealed that SDY had a modulatory effect on several processes related to host’s immunity, oxygen’s carrier capacity, sexual differentiation, metabolism, and digestion. This study supports the notion that brewery’s by-products like SDY are suitable for aquafeeds of carnivorous fish species such as the gilthead sea bream, and promotes a circular bioeconomy model that reuses, recovers and recycles resources instead of producing wastes
Project description:Sparicotylosis is an endemic parasitic disease across the Mediterranean Sea caused by the polyopisthocotylean monogenean Sparycotyle chrysophrii, which affects the gills of gilthead sea bream (Sparus aurata). Current disease-management, mitigation and treatment strategies are scarce against sparicotylosis. In order to successfully develop more efficient therapeutic strategies against this disease, understanding which molecular mechanisms and metabolic pathways are altered in the host is critical. This study aims to elucidate how S. chrysophrii infection modulates giltheadd seea bream physiological status and to identify the main altered biological processes through plasma proteomics of the host.
Project description:In this study, gilthead sea bream (Sparus aurata) fast muscle myoblasts were stimulated with two pro-growth treatments, amino acids (AA) and insulin-like growth factor 1 (Igf-1), to analyze the transcriptional response of genes, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) and their regulatory network. AA had a higher impact on gene transcription (1795 genes significantly changed) compared to Igf-1 (385 genes significantly changed). Both treatments stim-ulated the transcription of genes related to muscle differentiation (GO:0042692) and sarcomere components (GO:0030017), but AA stimulated more the DNA replication and cell division (GO:0007049). Notably, four miRNAs (miR-21, miR-146, miR-22b and miR-206) dominated the landscape among 403 expressed miRNAs. Both pro-growth treatments altered the transcription of over 100 miRNAs, including muscle-specific miRNAs (myomiRs) such as miR-133a/b, miR-206, miR-499, miR-1, and miR 27a. Among 111 detected lncRNAs (> 1 FPKM), only 30 were significantly changed by AA and 11 by Igf-1. Eight lncRNAs exhibited strong negative correlations with several mRNAs, suggesting direct regulation; while 30 lncRNAs showed strong correlations and interac-tions with several miRNAs, suggesting their function as miRNA’s sponges. This work is the first step in the identification of ncRNAs network controlling muscle development and growth in gilthead sea bream, pointing out potential regulatory mechanisms in response to pro-growth signals.
