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DYRK2 knockout mice exhibit fetal growth retardation

ABSTRACT: DYRK2 knockout mice exhibit fetal growth retardation

PROVIDER: PRJNA611501 | ENA |

REPOSITORIES: ENA

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DYRK2 knockout mice exhibit fetal growth retardation

Project description:We generated DYRK2-deficient mice using the CRISPR/Cas9 genome editing method and demonstrated that loss of DYRK2 gene causes fetal growth retardation and neonatal lethality at birth. Total RNA from DYRK2-/- whole embryo was compared with those of WT mice by microarray.

2020-04-27 | GSE146614 | GEO

Slc7a7 disruption causes fetal growth retardation by down-regulating Igf1 in the mouse model of LPI

Project description:The solute carrier family 7A member 7 gene (SLC7A7) encodes the light chain of the heterodimeric carrier responsible for cationic amino acid (CAA) transport across the basolateral membranes of epithelial cells in intestine and kidney. Mutations affecting SLC7A7 cause lysinuric protein intolerance (LPI), a multiorgan disorder with clinical symptoms that include visceromegaly, growth retardation, osteoporosis, hyperammonemia, and hyperdibasicaminoaciduria. Here, we describe the consequences of inactivating Slc7a7 in a mouse model of LPI. The Slc7a7 mutation was generated by highthroughput retroviral gene-trapping in embryonic stem cells. The Slc7a7-/- mouse displayed intrauterine growth restriction (IUGR), commonly leading to neonatal lethality. After heavy protein ingestion, the surviving adult animals presented metabolic derangement consistent with that observed in human LPI. IUGR was investigated by examining the expression of main factors controlling fetal growth. Insulinlike growth factor 1, the dominant fetal growth regulator in late gestation, was markedly downregulated as demonstrated by quantitative real-time RT-PCR, immunostaining and Western blot analysis in fetal liver. To further explore the pathophysiology of LPI, gene expression profiling analyses were carried out by DNA microarray technology in intestine and liver of adult Slc7a7-/- mice. Significant up-regulation or down-regulation (two-fold or greater) was observed for 488 transcripts in intestine, and for 521 transcripts in liver. The largest category of differentially expressed genes corresponds to those involved in transport according to Gene Ontology classification. This mouse model offers new insights into the pathophysiology of LPI and into mechanisms linking CAA metabolic pathways and growth control. KEYWORDS: cationic amino acid transport; hyperdibasicaminoaciduria; neonatal lethality. Keywords: Disease state analysis

2007-03-27 | GSE5432 | GEO

fetal growth restriction_pancreas_differences in nutritional condition

Project description:Quantification of proteins in the pancreas of fetal growth restriction.

2022-06-07 | PXD026502 | JPOST Repository

Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases

Project description:Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases

| PRJNA534258 | ENA

Severe damage on placental fetal capillary network causes mid to late fetal lethality and reduction of placental size in Peg11/Rtl1 KO mice [day 12.5]

Project description:Paternally expressed 11/Retrotransposon-like 1 (Peg11/Rtl1) knockout (KO) mice exhibited mid to late fetal lethality or late fetal growth retardation associated with frequent neonatal lethality. The lethal phenotype was largely dependent on genetic background and became more severe with each succeeding generation in the course of backcross experiments to C57BL/6 (B6). We previously suggested these lethal and growth phenotypes were due to severe defects in placental fetal capillaries in labyrinth layer. In this study, we reexamined KO fetuses exhibiting mid fetal lethality with internal bleeding. Importantly, basal region of fetal capillary network was specially damaged, therefore, also leading to poor expansion of the labyrinth layer and placental size reduction in later stage. Apparent up-regulation of Guanine nucleotide binding protein, alpha 2 (Gnai2) and decrement of Transmembrane protein 100 (Tmem100), Mesenchyme homeobox 2 (Meox2) and Lymphatic vessel hyaluronan Receptor 1 (LYVE1) expression were observed in earlier stage of placentas even before apparent morphological changes occurred, suggesting that these genes are involved in the maintenance of fetal capillaries associated with Peg11/Rtl1 in development.

2016-10-19 | GSE88891 | GEO

Severe damage on placental fetal capillary network causes mid to late fetal lethality and reduction of placental size in Peg11/Rtl1 KO mice [day 10.5]

2016-10-19 | GSE88849 | GEO

Mental Retardation Patients

Project description:Profiling the genomic profiles of mental retardation patients. 13 mental retardation patients were selected for detection of genomic aberrations.

2009-12-16 | GSE19457 | GEO

Real-time quantitative PCR analysis of jejunal and ileal mucosa from postnatal growth retardation and healthy pigs

Project description:The purpose of this study was to investigate the relative mRNA expression related to hormone, antioxidant capacity and immune responses in jejunal and ileal mucosa of healthy and postnatal growth retardation pigs. At 42 d of age, after overnight fasting, six postnatal growth retardation pigs and six healthy pigs were pair-matched by litter were selected for sampling. Samples of the jejunal and ileal mucosa were scraped and immediately snap-frozen in liquid nitrogen and stored at −80°C for RNA extraction. We used Roche LightCycler ® 480 Instrument PCR assay panel to quantitate gene expression of hormone, antioxidant capacity and immune responses relevant genes from jejunal and ileal mucosa.

2019-09-04 | GSE136777 | GEO

Identification and Comparative Analyses of Myocardial miRNAs Involved in Fetal Response to Maternal Undernutrition

Project description:Poor maternal nutrition during pregnancy causes intrauterine growth retardation, which, in turn, is associated with increased risk of cardiovascular and metabolic diseases in later life Fetal hearts were collected from baboon fetuses born to regularly fed and undernurished mothers. Total RNA was isolated, and fetal cardiac miRNA were profiled

2015-11-02 | E-GEOD-69609 | biostudies-arrayexpress

Transcriptomic analysis of colonic epithelial cells in adult rats born with intra-uterine growth retardation.

Project description:The impact of intra-uterine growth retardation (IUGR) was analyzed on gene expression of the colonic epithelial cells from adults rats fed a standard or a high-fat diet. The hypothesis tested in this study was that IUGR induces sustainable effects on colonocyte transcriptome that may alter further nutritional adaptations at adulthood, especially to high-fat diet.

2015-11-01 | GSE65051 | GEO

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