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MiCLIP-seq of postnatal day 0 (P0) normal mouse skin epithelial cells

ABSTRACT: miCLIP-seq of postnatal day 0 (P0) normal mouse skin epithelial cells

PROVIDER: PRJNA614972 | ENA |

REPOSITORIES: ENA

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miCLIP-seq of postnatal day 0 (P0) normal mouse skin epithelial cells

Project description:We employed miCLIP-seq to profile the location and extent of m6A in the Poly(A)+ transcriptome. Skin epithelial cells were isolated from wild-type P0 neonates by FACS. Total RNA was extracted by TRIzol-LS and Poly(A)+ RNA was extracted with Dynabeads™ mRNA Purification Kit (Thermo-Fisher, 61006). Input and miCLIP libraries was prepared from 3 biological replicates with each containing RNA isolated from 3 litters of neonates. Libraries were sequenced on the Illumina Hi-seq platform to generate paired-ended 50 bp reads. Sequencing data was processed as described in (Geula et al., 2015).

2020-09-04 | GSE147489 | GEO

RNA-seq of postnatal day 0 (P0) wild-type and Fezf2-/- cortices

Project description:The goal of the study was to compare gene expression of P0 wild-type and P0 Fezf2-/- cortices. Total RNAs were isolated from P0 cortices dissected from wild-type and Fezf2-/- mice (n=3 for each genotype), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA TruSeq library preparation guide.The libaries were pair-end sequenced (50nt per end). Differentially expressed genes were identified by DESEQ.

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RNA-seq of postnatal day 0 (P0) wild-type and Satb2-/- cortices

Project description:The goal of the study was to compare gene expression of P0 wild-type and P0 Satb2-/- cortices. Total RNAs were isolated from P0 cortices dissected from wild-type and Satb2-/- mice (n=3 for each genotype), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA TruSeq library preparation guide.The libaries were pair-end sequenced (50nt per end). Differentially expressed genes were identified by DESEQ.

2015-09-08 | GSE68911 | GEO

RNA-seq of postnatal day 0 (P0) wild-type and Satb2-/- cortices

2015-09-08 | E-GEOD-68911 | biostudies-arrayexpress

RNA-seq of postnatal day 0 (P0) wild-type and Fezf2-/- cortices

Project description:RNA-seq of postnatal day 0 (P0) wild-type and Fezf2-/- cortices

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RNA-seq of postnatal day 0 (P0) wild-type, Fezf2-/- cortices, and Fezf2-/-;Fezf2-EnR cortices

Project description:The goal of the study was to compare gene expression of P0 wild-type, P0 Fezf2-/- cortices, and Fezf2-/-; Fezf2-EnR cortices. Total RNAs were isolated from P0 cortices dissected from wild-type (n=3), Fezf2-/- mice (n=4), and Fezf2-/-; Fezf2-EnR cortices (n=2), following Qiagen RNAeasy kit instruction.Sequence libraries were made following Illumina RNA PolyA library preparation guide.The libaries were pair-end sequenced (150nt per end). Differentially expressed genes were identified by DESEQ.

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Effects of Adjudin on gene expression of postnatal day 0 mouse pancreatic islets

Project description:To investigate effects of Adjudin on gene expression of postnatal day 0 mouse islets (P0 islets), islets from postnatal day 0 mouse (regardless of sex) were isolated, cultured in incubator for overnight recovery, and treated with either DMSO or 10 µM Adjudin for 1 day before RNA sequencing.

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RNA-seq of postnatal day 0 (P0) wild-type, Fezf2-/- cortices, and Fezf2-/-;Fezf2-EnR cortices

Project description:RNA-seq of postnatal day 0 (P0) wild-type, Fezf2-/- cortices, and Fezf2-/-;Fezf2-EnR cortices

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P0 wild type tooth and skin

Project description:P0 whole tooth germ cells versus P0 skin cells

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Transcription profiling of early development of neural crest stem cells and developing Schwann cells, from embryonic day 9.5 to postnatal day 0.

Project description:Time course of early development of peripheral nerve, from embryonic day 9.5 to postnatal day 0. Origin of samples: mouse embryos expressing green fluorescent protein (GFP) in neural crest stem cells and later glial cells under the control of the proteolipid protein gene (Plp) promoter. At E9.5 the trunk region was dissected out. At E12, E14, E16, E18 and P0 the sciatic nerve was taken. GFP-expressing cells were isolated by FACS sorting. Three or 4 separate batches of embryos analyzed at each stage.

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