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RNA bisulfite sequencing for polysome fractions

ABSTRACT: RNA bisulfite sequencing for polysome fractions

PROVIDER: PRJNA625655 | ENA |

REPOSITORIES: ENA

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RNA 5'end sequencing in polysome profiling fractions

Project description:It has been shown that in mammalian cells alternative transcription initiation is extensively regulated during development and across cell-types, which confers dynamic transcript 5âUTR repertoire. However it is underexplored how the heterogeneity of 5âUTR isoforms would affect the downstream steps for protein expression, such as translation. To this end, we globally compared the translational profile of distinct mRNA TSS isoforms in mouse fibroblast cells, by combining deep-sequencing based mRNA 5âends profiling and polysome fractionation. We demonstrated the extensive translation regulation conferred by TSS heterogeneity. 5'end sequencing in seven polysome fractions, in two replicates, using Illumina Hiseq2000

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RNA bisulfite sequencing for polysome fractions

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Transcriptome of Trypanosome brucei after PUF2 Knock-down - polysome fractions

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