Ontology highlight
ABSTRACT:
PROVIDER: PRJNA637602 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Masson pine (Pinus massoniana) has evolved some adaptations for growth in low P soils. To elucidate these mechanisms, we investigated global gene expression profiles of the masson pine responding to long-term phosphorus starvation and different Pi levels (P1, 0.01 mM P; P2, 0.06 mM P).
2013-11-30 | GSE52835 | GEO
Project description:Masson pine (Pinus massoniana) has evolved some adaptations for growth in low P soils. To elucidate these mechanisms, we investigated global gene expression profiles of the masson pine responding to long-term phosphorus starvation and different Pi levels (P1, 0.01 mM P; P2, 0.06 mM P). Analysis used phosphorus-sufficient treatment RNA as control samples for comparison to the experimental samples (P1 and P2) taken at 12, 24, 48 and 60 day. Indirect comparisons were made across multiple arrays with raw data pulled from different channels for data analysis and comparison to the control data.
2013-11-30 | E-GEOD-52835 | biostudies-arrayexpress
Project description:Pine wilt disease is a worldwide dangerous pine disease. We used Masson pine (Pinus massoniana) clones, selected through traditional breeding and testing for 20 years, with high resistance to study the molecular mechanism of resistance to pine wood nematode (PWN, Bursaphelenchus xylophilus). A total of 3491 proteins were identified from seedling tissue, among which 2783 proteins contained quantitative information. Total 42 proteins were up-regulated and 96 proteins were down-regulated in resistant lines. Of them, function enrichment analysis found that significant differences in proteins with pectin esterase activity or peroxidase activity. Proteins participating in salicylic acid metabolism, antioxidant stress reaction, polysaccharide degradation, glucose acid ester sheath lipid biosynthesis, sugar glycosaminoglycans degradation pathway also changed significantly. PRM results showed that pectin acetyl esterase, carbonic anhydrase, peroxidase and chitinase were significantly down-regulated, while aspartic protease was significantly up-regulated, which was consistent with proteomic data.These results suggested that Masson pine could degrade nematode-related proteins by increasing protease to inhibit their infestation, and enhance the resistance of Masson pine to PWN by down-regulating the carbon metabolism to limit available carbon to PWN or to be involved in cell wall components or tissue softening. Most downregulated proteins seem to take back seats prior to pathogen attacks. The highly resistant Masson pine, very likely, has evolved multiple pathways, both the passive and active, to defense against PWN infestation.
2022-10-13 | PXD030664 | Pride
Project description:Wood stiffness is the most important wood quality trait of forest trees for structural timber production. We investigated genes differentially transcribed in radiate pine trees with distinct wood stiffness using bulked segregant analysis (BSA) and cDNA microarrays. Transcript accumulation in earlywood (EW) and latewood (LW) of high (HS) and low stiffness (LS) trees in two progeny trials was compared. Radiata pine trees used for microarray experiment were selected from two progeny trials planted at Flynn and Kromelite, Australia. Based on the IML-based MOE measurement, five families with highest and lowest MOE each were selected from each trial, which represented two segregant populations with contrasting wood stiffness. Two individuals from each selected family were further sampled. Developing xylem tissues of selected trees in Flynn trial were sampled in spring (October) and autumn (April), representing earlywood (EW) and latewood (LW) of juvenile aged trees, respectively. Collection of xylem tissues from Kromelite trial was arranged in summer (late November) when latewood (LW) was formed. The xylem tissues were scraped at breast height with a sharp chisel after the bark was removed. In Flynn trial EW and LW tissues were collected from the same sampled trees on opposite sides of the trunk. Transcript accumulation was compared in trees with highest (HS) and lowest stiffness (LS) using xylem samples from Flynn collected in spring (EW) and autumn (LW), as well as Kromelite in summer (LW), respectively. Bulked segregant analysis (BSA) was used for the experiment design. Total RNA samples extracted from the five trees with HS were pooled at equal amount, and compared to the bulked five individuals with LS. This pooling strategy can partly minimize the genetic variation among different genotypes. Dye swaps were applied in each biological replicate.
2011-06-01 | E-GEOD-23020 | biostudies-arrayexpress
Project description:The meristem-associated endosymbiont M. extorquens DSM13060 significantly increases needle and root growth of Scots pine (Pinus sylvestris L.) seedlings without producing plant hormones, but by aggregating around host nuclei. Here we studied gene expression of the pine host induced by M. extorquens DSM13060 infection. We selected the time point of 90 days post-inoculation for our analysis based, because at this point, Methylorubrum extorquens DSM13060 has systemically colonized the pine seedlings, being found throughout tissues of roots and shoots.
2021-04-15 | GSE170995 | GEO