Project description:HSFA9 (Medtr4g126070) is a seed-specific heat shock transcription factor that is upregulated during the later stages of seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Tnt1 insertion mutants were identified and RNA sequencing was performed on mature seeds from two hsfa9 mutants and the wild type seeds.
Project description:ABI4 (MtrunA17_Chr5g0437371) is an AP2 transcription factor that is upregulated during seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Tnt1 insertion mutants were identified and RNA sequencing was performed on two abi4 mutants and the wild type seeds at 13 DAP, 24 DAP and mature seeds.
Project description:ngs2016_10_dormancy - RNA-Seq on mpk8 and tcp14 mutants on dry and imbibited seeds-Comparison of the expression of genes in wild seeds and in mpk8 and tcp14 mutants on dry seeds and at a precise imbibition time (24h, 25°C) and in the dark.-Dry harversted seed: WT, mpk8, tcp14, 24h of imbibition in dark at 25°C: WT, mpk8, tcp14.
Project description:L. pneumophila Lp02 (thyA hsdR rpsL; MB110) is a virulent thymine auxotroph derived from the Philadelphia 1 clinical isolate and was the parental strain for all constructed mutants. To construct a letS (lpg1912) insertion mutant that lacks pflaG, the letS locus containing the transposon insertion was amplified from MB417 and transferred to Lp02 by natural competence, resulting in strain MB416 (27). Within each experiment, similar culture densities were used to analyze strain phenotypes.
Project description:We identified a regulator of Arabidopsis thaliana seed germination: FLOE1 (AT4G28300). We used RNA-seq to uncover genes that are differentially regulated in dry seeds, imbibed seeds and seeds imbibed in 220mM NaCl in mutant lines complemented with a ΔDS deletion version of FLOE1 (ΔDS) compared to mutants complemented with a WT version of FLOE1 (+WT).
Project description:Whole-genome resequencing of eight transcription factor mutants and one wild-type, in order to verify the T-DNA insertion site and its uniqueness.
Project description:We used transposon insertion sequencing (Tn-Seq) to identify the genes that are required for in vitro growth and intramacrophage growth of the live vaccine strain of F. tularensis (LVS).