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Transcriptome information of RAW-Rv3722c and RAW-Vector cell lines

ABSTRACT: Transcriptome information of RAW-Rv3722c and RAW-Vector cell lines

PROVIDER: PRJNA661267 | ENA |

REPOSITORIES: ENA

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Transcriptome information of RAW-Rv3722c and RAW-Vector cell lines

Project description:RAW-Rv3722c and RAW-Vector cells were collected for RNA extraction and subject to transcriptome sequencing. Expression levels of all genes in the two cell lines were determined by Next Generation Sequencing (NGS)

2021-01-01 | GSE157419 | GEO

Transcriptome profiling of HCC1937 vector and wtBRCA1 cell lines which were treated with OTX-015

Project description:Purpose: To compare the transcriptomes of HCC1937 vector and wtBRCA1 cells which were treated with OTX-015. Methods: HCC1937 vector and wtBRCA1 cells were treated with 5 micromolar OTX-015 for 24 hours. Total RNA was extracted and processed with NEBNext Ultra Directional RNA Library Prep Kit for cDNA library preparation. The data are in duplicate and raw data were generated by Illumina 2500 sequencer. The Fastq format data were then processed with Tophat for genome mapping. After getting the bam files, they were further analyzed with cuffdiff for gene expression of different groups. Results: From the transcriotome profiling and analysis, we got gene expression data of DMSO control group and OTX-015 treatment group for the HCC1937 BRCA1 isogenic cell lines. Conclusion: The gene expression data revealed the transcriptome variation by OTX-015 treatment.

2019-01-30 | GSE109809 | GEO

Transcriptome profiling of HCC1937 vector and wtBRCA1 cell lines which were treated with OTX-015

Project description:Transcriptome profiling of HCC1937 vector and wtBRCA1 cell lines which were treated with OTX-015

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Gene expression in ABHD5-KD RAW cells or RAW cell-primed CT-26 cells

Project description:RAW cells,ABHD5-KD RAW cells or those macrophage-primed CT-26 cells were subjected to microarray assays.Each group contain 3 samples.

2019-08-11 | GSE135673 | GEO

Expression data of PRDM1α or empty vector-transduced NK92 or KHYG1 cell lines

Project description:Expression data of PRDM1α or empty vector-transduced NK92 or KHYG1 cell lines

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Vector

Project description:Vector Targeted Locus (Loci)

| PRJNA315083 | ENA

Genome-wide maps of gV sPLA2 binding sites in Raw 264.7 cells

Project description:We have searched genome-wide binding sites of mouse group V secretory phospholipase A2 (gV sPLA2) in Raw 264.7 cells. Since we previously had technical problems in immunoblotting analysis and immunoprecipitation of the gV sPLA2 protein due in part to unavailability of a specific antibody against mouse gV sPLA2 and other issues, we used a monoclonal antibody against the Myc-tag to analyze gV sPLA2 knockdown (KD) RAW 264.7 cells with re-constitutive expression of Myc-tagged gV sPLA2 or Myc-tagged gV sPLA2-H48Q, lacking catalytic activity, as well as gV sPLA2 KD RAW 264.7 cells transfected with empty vector. Among the significant peaks, the region corresponding to -375 ~ +6 from the transcriptional start site of the Pgk1 (phosphoglycerate kinase 1) gene was the strongest binding peak in the nucleus of both gV sPLA2 KD cells expressing Myc-tagged gV sPLA2 and gV sPLA2 KD cells expressing Myc-tagged gV sPLA2-H48Q. Meanwhile, the Pgk1 gene was not identified as a significant peak in the nucleus of gV sPLA2 KD cells transfected with empty vector.

2021-03-26 | GSE156528 | GEO

miRNAs expression in HepG2 hepatocellular carcinoma cell lines: Vector Control vs. Twist1, Bcl-2, and Twist1/Bcl-2 Transfected

Project description:miRNAs profiling of HepG2 cells comparing vector-control treated HepG2 cells with HepG2 cells transfected with Twist1, Bcl-2, and Twist1/Bcl-2 plasmids. Microarray analysis revealed a panel of miRNAs with significant differential expression among these four HCC cell lines.

2017-02-10 | GSE77501 | GEO

Mammalian cell lines

Project description:Mammalian cell lines Raw sequence reads

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mRNA Profiling of miR-17 family inhibition using TuD lentiviral vector in HepG2 and SK-Hep1 hepatocellular carcinoma cell lines [RNA-Seq]

Project description:To functionally characterize the role of miR-17 family in HCC, lentiviral vector-based miR inhibitor TuD was used to inhibit miR-17 family of microRNAs in HepG2 and SK-Hep1 HCC cell lines

2017-06-01 | GSE93831 | GEO

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