Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of mouse bone marrow dendritic cells

ABSTRACT: RNA sequencing of mouse bone marrow dendritic cells

PROVIDER: PRJNA666548 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing of mouse bone marrow dendritic cells

Project description:Bulk mRNA from mouse bone marrow-derived dendritic cells (BMDCs) was sequenced. Samples were obtained from Wild Type (C57BL/6J) mice, or mice with GNAS knocked out specifically in CD11c +ve cells.

2020-10-01 | GSE158783 | GEO

PU.1 interactome mouse bone marrow derived dendritic cells

Project description:Mouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected at day 10. 2x10E8 cells were used to prepare whole cell extracts and to perform PU.1 immunoprecipitaion with PU.1 antibody (T-21 Santa Cruz). IgG was used as control.

2018-11-30 | MSV000083176 | MassIVE

RNA Sequencing Analysis of Mouse Bone Marrow Derived Dendritic Cells Transcriptomes During Maturation

Project description:Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell maturation. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse GM-CSF and IL-4, mature DCs were obtained after LPS induced maturation. Immature DCs and mature DCs were sorted respectively based on maturation marker CD86 and Iab(MHCII) using flowcytrometer. DC mRNA profiles were generated by deep sequencing,using Illumina Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified 1,300 upregulated genes and 1,475 dow regulated genes during dendritic cell maturation. DC mRNA profiles immature and mature moouse BMDCs were generated by deep sequencing

2015-03-04 | E-GEOD-60307 | biostudies-arrayexpress

Expression data from mouse bone marrow myeloid dendritic cells (classical DCs) and classical dendritic cells in spleen

Project description:Classical dendritic cells may be found in mouse bone marrow and spleen. Due to the differences in their local environment, two populations may express different genes and potentially carry different functions We used microarrays to compare the gene expression profiles between myeloid dendritic cells and classical dendritic cells in spleen. Our data supported the hypothesis that bone marrow myeloid dendritic cells are enriched for the expression of certain sets of genes that may play specific functions in the bone marrow microenvironment

2018-03-21 | GSE112082 | GEO

RNA Sequencing Analysis of Mouse Bone Marrow Derived Dendritic Cells Transcriptomes During Maturation

2015-03-04 | GSE60307 | GEO

Mouse bone marrow-derived dendritic cell differentiation in dense and sparse cultures

Project description:The aim of our experiments was to identify the influence of bone marrow cell culture concentration on gene expression profile of developing mouse bone marrow dendritic cells.

2017-03-14 | E-MTAB-4614 | biostudies-arrayexpress

Mouse bone marrow inDrop

Project description:Mouse bone marrow inDrop

| PRJNA432520 | ENA

Bone marrow dendritic cell and exosome RNA profiling

Project description:In this study, we sought to identify differentially RNAs found in mouse bone marrow-derived dendritic cells and their exosomes. As a secondary aim, we sought to determine whether deletion of the Sidt1 and Sidt2 genes affects the RNA content of exosomes.

2021-09-02 | GSE183258 | GEO

Bone marrow dendritic cell and exosome RNA profiling

Project description:Bone marrow dendritic cell and exosome RNA profiling

| PRJNA759845 | ENA

Chronic Rapamycin treated bone marrow dendritic cells

Project description:Chronic Rapamycin treated bone marrow dendritic cells

| PRJNA664129 | ENA