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Interactions between Verticillium longisporum and plant terpenes

ABSTRACT: Interactions between Verticillium longisporum and plant terpenes

PROVIDER: PRJNA667088 | ENA |

REPOSITORIES: ENA

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Interactions between Verticillium longisporum and plant terpenes

Project description:Plant roots secrete secondary metabolites to sense the enviroment around them. Among them, terpenes play a prominent role. Terpenes can have either fungistatic or fungicide action. However, their exact role in plant-host interactions is not fully understood. Verticillium longisporum is a soilborne pathogen causing disease in Brasicacae plants. In this project we investigated the transcriptomic changes of this species upon exposure to the β-pinene monoterpene in different time points 0hpi, 8hpi, 24hpi and 24hpi.

2021-11-07 | GSE158956 | GEO

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Project description:Identification of genes differentially expressed in roots of Arabidopsis Col-0 and ndr1-1 mutants 48 h post inoculation with the fungal pathogen Verticillium longisporum.

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V. longisporum elicits media-dependent secretome responses with a further capacity to distinguish between plant-related environments

Project description:Plant pathogens secrete distinct effector molecules in their host plants to establish successful infections. Verticillium longisporum colonizes the vascular system of the plant. We analyzed the exoproteome of the fungus dependent on different environmental conditions including non plant-related and plant-related environments. This exoproteomic comparison led to the identification of a set of proteins that are specifically secreted in the xylem sap.

2020-07-27 | PXD018533 | Pride

Expression data roots of Arabidopsis plants inoculated with Verticillium longisporum

Project description:Identification of genes differentially expressed in roots of Arabidopsis Col-0 and ndr1-1 mutants 48 h post inoculation with the fungal pathogen Verticillium longisporum. Arabidopsis Col-0 and ndr1-1 seeds were grown in petri dishes with liquid MS medium supplemented with 0.2% sucrose. After two weeks on a horizontal shaker (40 rpm), plants were rinsed twice in sterile distilled water and transferred to liquid 0.5xMS medium without sucrose, supplemented with 200 conidia ml-1 of V. longisporum isolate VL1 (CBS110220). Control plants were grown in 0.5x MS only. At 48 hours post inoculation, materials were harvested in biological replicates (>50 plant roots) and immediately frozen in liquid nitrogen. RNA was extracted with Aurum Total RNA Mini Kit (Bio-Rad) and hybridized to the Arabidopsis ATH1 Genome Array at the DNAVision facility (Gosselies, Belgium). Microarray assays were performed with triplicate biological root samples. Normalization of the microarray data was performed using the Robust Multichip Average (RMA) method as implemented by the “quantile normalization” function in the Bioconductor 1.3 software.

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