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Comparative transcriptome analyses of the Drosophila pupal eye

ABSTRACT: Comparative transcriptome analyses of the Drosophila pupal eye

PROVIDER: PRJNA673208 | ENA |

REPOSITORIES: ENA

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Comparative transcriptome analyses of the Drosophila pupal eye

Project description:Investigating Drosophila melanogaster genome-wide gene expression differences in developing pupal eyes comparing 21 hours After puparium formation (APF) to 40 hours APF.

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Expression data from Control and Yorkie overexpression pupal eye discs

Project description:To probe mechanistic determinants of Yki function in mitochondrial biogenesis, we conducted a genome-wide microarray experiment, and specifically compared expression patterns of genes from control (GMR gal4) and Yorkie over-expressing (GMR gal4; UAS yki) pupal eye discs. The above mentioned genotypes were grown at 29 deg C and at about 40 hrs after pupariation, the pupal eye discs were dissected. RNA was extracted from the pupal eye discs, purified and used to generate microarray probes that were hybridized to the Drosophila genome 2 arrays (Affymetrix). The Gene Chip Operating system (Affymetrix) and dCHIP program (Harvard University) were used to generate pairwise comparisons between the transcription profiles of control and Yorkie over-expressing discs.

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Comparative transcriptome analyses of the Drosophila pupal eye.

Project description: Not available

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Project description:We exploit the predictable time course of Drosophila brain development to perform a temporally coupled quantitative proteomic analysis of the pupal brain in Nab2 mutant or overexpression models, which reveals that Nab2 is required to regulate the abundance of a number of proteins with critical roles in Drosophila neurons. Pupal brains lacking Nab2 show dysregulation of proteins, such as Futsch, Turtle, Contactin, and Van Gogh, that typically function in brain morphogenesis, neuroblast proliferation, circadian sleep/wake cycle, and other neurodevelopmental processes. Overall, these data define a role for Nab2 during neurodevelopment in regulating protein abundance for a subset of the brain proteome and provide a window into the potential functions of human ZC3H14 protein.

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Expression data from Control and Yorkie overexpression pupal eye discs

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Transcription profiling of Drosophila wild type Canton S vs. otd-uvi eye specific mutant alles of Otd revals regulation of gene networks in photoperception-related functions by the CRX/OTX homologue

Project description:Comparative global gene expression analysis was caried out using pupal head tissue of otd-uvi an eye specific mutant allele of Otd and wild type control Canton S., in order to delineate the function of homeobox transcription factor Otd in Drosophila photoreceptor development and function. Experiment Overall Design: The Drosophila strain Canton S was used as wild type for comparative gene expression analysis with the mutant strain otd-uvi. For each biological replicate, samples were derived from independent RNA extractions consisting of 15-20 pupal heads. Pupae were dissected at 80 hr APF and immediately transferred to 600ul of trizol (GIBCO/BRL). RNA extraction was performed according to manufacturers instructions. Qualitative and quantitative analysis of the total RNA was carried out by capillary electrophoresis on a RNA6000 Nano Assay chip, with Agilent 2100 Bioanalyzer (Agilent Technologies USA) Biotin labeled probes were generated from 10ug total RNA according to Affymetrix protocol.

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Regulation of gene networks in photoperception-related functions by the CRX/OTX homologue Otd in Drosophila

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Drosophila Eye Development - Larval Stage

Project description:Analysis of gene expression during Drosophila eye formation. Keywords: other

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An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia

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