Transcriptomics,Genomics

Dataset Information

161

Transcriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous system


ABSTRACT: Transcriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement of neuronal SOCE for Drosophila flight. We identified the early pupal stage to be critical and used RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous system. We down-regulated dStim, the endoplasmic reticular calcium sensor and a principal component of SOCE in the nervous system for a 24h period during pupal development, and compared wild type and knockdown transcriptional profiles, immediately after knockdown as well as after a 36h recovery period. We found that dStim knockdown altered the expression of a number of genes. We also characterized one of the down-regulated genes, Ral for its role in flight. Thus, we identify neuronal SOCE as a mechanism that regulates expression of a number of genes during the development of the pupal nervous system. These genes can be further studied in the context of pupal nervous system development. Overall design: mRNA sequencing from two biological replicates each of wild type and dStim knockdown pupal brains at two time points - 36h APF (post 24h knockdown) and at 72h APF (Post knockdown and recovery)

REANALYSED by: GSE117217

INSTRUMENT(S): Illumina HiSeq 2000 (Drosophila melanogaster)

SUBMITTER: Gaiti Hasan  

PROVIDER: GSE89168 | GEO | 2017-03-21

SECONDARY ACCESSION(S): PRJNA350516

REPOSITORIES: GEO

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Publications

A pupal transcriptomic screen identifies Ral as a target of store-operated calcium entry in Drosophila neurons.

Richhariya Shlesha S   Jayakumar Siddharth S   Abruzzi Katharine K   Rosbash Michael M   Hasan Gaiti G  

Scientific reports 20170214


Transcriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement for SOCE in neurons that regulate Drosophila flight bouts. We refine this requirement temporally to the early pupal stage and use RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous s  ...[more]

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