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Drosophila melanogaster

ABSTRACT: Transcriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous system

PROVIDER: PRJNA350516 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4449930.fastq.gz	Fastqsanger.gz
	SRR4449931.fastq.gz	Fastqsanger.gz
	SRR4449932.fastq.gz	Fastqsanger.gz
	SRR4449933.fastq.gz	Fastqsanger.gz
	SRR4449934.fastq.gz	Fastqsanger.gz

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Transcriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous system

Project description:Transcriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement of neuronal SOCE for Drosophila flight. We identified the early pupal stage to be critical and used RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous system. We down-regulated dStim, the endoplasmic reticular calcium sensor and a principal component of SOCE in the nervous system for a 24h period during pupal development, and compared wild type and knockdown transcriptional profiles, immediately after knockdown as well as after a 36h recovery period. We found that dStim knockdown altered the expression of a number of genes. We also characterized one of the down-regulated genes, Ral for its role in flight. Thus, we identify neuronal SOCE as a mechanism that regulates expression of a number of genes during the development of the pupal nervous system. These genes can be further studied in the context of pupal nervous system development.

2017-03-21 | GSE89168 | GEO

Chronic dysfunction of Store-operated calcium entry by pulsed RNAi induction in fat tissue impairs organismal energy homeostasis in Drosophila

Project description:The purpose is to have a overview on how chronic dysfunction of store-operated calcium entry change transcription profiles of adult fly fat tissue. Methods:To achieve chronic dysfunction of store-operated calcium entry, we carried out the Temperature-caused RNAi Pulse Induction of Stim (29 celcius degree incubation, Stim-TRiPI, or Stim-TRIP in data file) in fat storage tissue of 6 days old adult male flies. At day 10 and 11 after Stim-TRiPI (Stim-TRIP), we isolated the fat tissues and carried out RNAseq analysis based gene-level read counts.

2019-05-07 | GSE112689 | GEO

Selective modulation of gene expression in activated normal human peripheral blood mononuclear cells by store-operated calcium entry blocker BTP2

Project description:Calcium is a critical signaling molecule in many cell types including immune cells. The calcium-release activated calcium channels (CRAC) responsible for store-operated calcium entry (SOCE) in immune cells are gated by STIM family members functioning as sensors of Ca2+ store content in the endoplasmic reticulum. We investigated the effect of SOCE blockers BTP2 and CM4620 on human peripheral blood mononuclear cells (PBMCs) polyclonally-stimulated with the mitogen phytohemagglutinin (PHA).

2025-04-28 | GSE295784 | GEO

Selective modulation of gene expression in activated normal human peripheral blood mononuclear cells by store-operated calcium entry blocker BTP2

Project description:Calcium is a critical signaling molecule in many cell types including immune cells. The calcium-release activated calcium channels (CRAC) responsible for store-operated calcium entry (SOCE) in immune cells are gated by STIM family members functioning as sensors of Ca2+ store content in the endoplasmic reticulum. We investigated the effect of SOCE blocker BTP2 on human peripheral blood mononuclear cells (PBMC) stimulated with the mitogen phytohemagglutinin (PHA).

2023-08-07 | GSE226570 | GEO

saraf

Project description:Danio rerio saraf, store-operated calcium entry-associated regulatory factor [Source:NCBI gene;Acc:767649], is expressed in 1 baseline experiment(s);

| ENSDARG00000070452 | ExpressionAtlas

Saraf

Project description:Rattus norvegicus Saraf, store-operated calcium entry-associated regulatory factor [Source:RGD Symbol;Acc:1303011], is differentially expressed in 23 experiment(s);

| ENSRNOG00000012329 | ExpressionAtlas

Saraf

Project description:Rattus norvegicus Saraf, store-operated calcium entry-associated regulatory factor [Source:RGD Symbol;Acc:1303011], is expressed in 7 baseline experiment(s);

| ENSRNOG00000012329 | ExpressionAtlas

Differential regulation of membrane potential during store-operated calcium entry in neutrophil subsets

Project description:Calcium signals are initiated in immune cells by the process of store-operated calcium entry (SOCE), where receptor activation triggers transient calcium release from the endoplasmic reticulum, followed by opening of plasma membrane calcium-release activated calcium (CRAC) channels. ORAI1, ORAI2 and ORAI3 are known to comprise the CRAC channel, however the contributions of individual isoforms to neutrophil function is not well understood. Here we show that loss of ORAI1 partially decreases calcium influx while loss of both ORAI1 and ORAI2 completely abolishes store-operated calcium entry. In other immune cell types, loss of ORAI2 enhances SOCE. In contrast, we find that ORAI2-deficient neutrophils display decreased calcium influx, which is correlated with measurable differences in regulation of neutrophil membrane potential via KCa3.1. Decreased SOCE in ORAI1-, ORAI2- and ORAI1/2-deficient neutrophils impairs multiple neutrophil functions including phagocytosis, degranulation, leukotriene and ROS production, rendering ORAI1/2-deficient mice highly susceptible to staphylococcal infection. This study demonstrates that ORAI1 and ORAI2 are the primary components of the neutrophil CRAC channel and identifies novel subpopulations of neutrophils where cell membrane potential functions as a rheostat to modulate the SOCE response. These findings have implications for new mechanisms that modulate neutrophil function during infection, acute and chronic inflammatory conditions, and cancer.

2020-09-01 | GSE157200 | GEO

ENSOARG00000000541

Project description:Ovis aries ENSOARG00000000541, store-operated calcium entry regulator STIMATE [Source:NCBI gene (formerly Entrezgene);Acc:101108428], is expressed in 2 baseline experiment(s);

| ENSOARG00000000541 | ExpressionAtlas

SARAF

Project description:Sus scrofa SARAF, store-operated calcium entry associated regulatory factor [Source:VGNC Symbol;Acc:VGNC:96187], is expressed in 4 baseline experiment(s);

| ENSSSCG00000033402 | ExpressionAtlas

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