Project description:Identification of the DNA binding landscape of the transcription factor regulatory factor X 7 (RFX7) in Nutlin-3a and DMSO control treated HCT116 cells.

2021-06-17 | GSE162157 | GEO

RFX7, ANKRA2, and p53-regulated transcriptome in RPE-1

Project description:Identification of the RFX7-regulated transcriptome in Nutlin-3a and DMSO control treated RPE-1 cells

2021-06-20 | GSE162161 | GEO

RFX7 DNA binding landscape in HCT116

Project description:RFX7 DNA binding landscape in HCT116

| PRJNA680775 | ENA

RFX7, ANKRA2, and p53-regulated transcriptome in RPE-1

Project description:RFX7, ANKRA2, and p53-regulated transcriptome in RPE-1

| PRJNA680778 | ENA

The transcription factor Rfx7 limits metabolism of NK cells promoting their maintenance and immunity

Project description:Regulatory factor X 7 (Rfx7) is an uncharacterized member of a family of transcription factors playing essential functions in ciliogenesis and immunity. Given the prominent expression of Rfx7 in lymphoid organs, we generated mice with Rfx7-deletion in the hematopoietic compartment. We assessed the function of Rfx7 in natural killer cells, which are innate lymphoid cells that protect from viral infections and cancer. By comparing the transcriptome of Rfx7-deficient and control NK cells isolated from bone marrow and spleen, we identified negative regulators of cell metabolism among the genes regulated by this transcription factor.

2018-06-26 | GSE113267 | GEO

RFX7 DNA binding landscape in U2OS

Project description:Identification of the DNA binding landscape of the transcription factor regulatory factor X 7 (RFX7) in Nutlin-3a and DMSO control treated U2OS cells.

2021-06-17 | GSE162158 | GEO

Integrated Omics Profiling Identifies Hypoxia-Regulated Genes in HCT116 Colon Cancer Cells

Project description:Hypoxia is associated with poor prognosis in most solid tumors due to its multiple effects on therapy resistance, angiogenesis, apoptotic resistance, and tumor invasion/metastasis. Here we used a comprehensive omics profiling to investigate hypoxia-regulated gene expression in HCT116 colon cancer cells. Quantitative analyses of proteome and secretome were performed in HCT116 cells cultured under hypoxic or normoxic conditions. A total of 5,700 proteins were quantified in proteome analysis and 722 proteins were quantified in secretome analysis. Both datasets were combined with the transcriptome and translatome datasets for further analysis. Verification of candidate proteins/genes in this integrated omics analysis was performed using immunoblotting and quantitative real-time RT-PCR analyses. We also performed polysome profiling to assess changes in translational efficiency of hypoxia-induced genes. Notably, several genes were differently regulated at the transcriptional and translational levels in HCT116 cells during hypoxia. Bioinformatics analysis suggested that hypoxia regulates translation of genes involved in extracellular matrix organization, extracellular exosomes, and protein processing in endoplasmic reticulum. Aberrations in these metabolic pathways appear to be correlated with an increased risk of tumor invasion/metastasis.

2018-03-14 | PXD009009 | Pride

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