Ontology highlight
ABSTRACT:
PROVIDER: PRJNA682485 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Apicomplexan parasites cause numerous diseases in humans and animals, including malaria (Plasmodium species) and toxoplasmosis (Toxoplasma gondii). Despite being a major drug target, the protein composition of the coenzyme Q:cytochrome c oxidoreductase complex (Complex III) in these parasites was previously unknown. Our work highlights the divergence of mitochondrial ETC Complex III composition in apicomplexan parasites and provides a broader understanding of Complex III evolution. Our study also provides important insights into what sets this major drug target apart from the equivalent complex in host species. Future studies can build on our findings to reveal how novel subunits contribute to Complex III function to further investigate this important drug target.
2021-01-27 | PXD018781 | Pride
Project description:Kinetochores are multiprotein assemblies that bind chromosomes to the spindle during mitosis. Despite conserved roles, repertoires of kinetochore proteins vary greatly across eukaryotes. In particular, most known components are not clearly detected in a group of parasites known as the Apicomplexa. Furthermore, flexibility in scale of amplification and an apparent incapability to delay cell cycle progression in response to spindle integrity has coined an idea these parasites divide in the absence of spindle checkpoints. In this study, we reunite divergent apicomplexan kinetochore components to a common eukaryotic set and additionally identify 9 kinetochore proteins that share little homology to known proteins. AKiTs are essential for chromosome segregation and show modes of division parallel to eukaryotic metaphase to anaphase transition and spindle assembly checkpoint signaling. These findings suggest conserved spindle assembly checkpoint signaling maintains fidelity during chromosome segregation in apicomplexan parasites.
2023-03-11 | PXD028595 | Pride
Project description:ApiAP2 are a family of conserved transcription factors (TF) that play an important role in regulating gene expression in apicomplexan parasites. ApiAP2 proteins that may control the cell cycle dependent expression program are to be discovered. To better understand how these cell-cycle dependent gene expression profiles are established, we identified ApiAP2 proteins showing a cell cycle dependent expression. Using the Auxin-degradation system, we produced several inducible knock-down (iKD) mutant for these ApiAP2 proteins. Among them, a cell-cycle regulated ApiAP2 is expressed during the late S-phase of the tachyzoite cell-cycle. The iKD mutant parasites for this gene are unable to proliferate. In presence of Auxin, the mutant parasites do not produce daughter cells and are arrested in the early phase of budding. This indicates that this TF may specifically regulate the early steps of the daughter cell formation. Using RNA-Seq, we demonstrate that the level of expression of number of transcripts is affected by the knock-down of this potential TF.
2020-10-29 | GSE150405 | GEO
Project description:Toxoplasma gondii is an apicomplexan parasite infecting human and animals, causing huge health concerns and economic losses. Calcium ion, a critical second messenger in cells, can regulate related vital activities, particularly in parasite invasion and escape processes. Calmodulin (CaM) is a short, highly conserved Ca2+ binding protein found in all eukaryotic cells, including apicomplexan parasites. After binding to Ca2+, CaM can be activated to interact with a variety of proteins (such as enzymes). Since direct destruction of CaM is impossible, few studies have been conducted on the function of CaM in T. gondii. We generated the CaM indirect knockout strain (iCaM) using a tetracycline-off system with CaM promoter sequence in T. gondii TATI strain, and compared the transcriptomes of tachyzoites with and without Calmodulin.
2022-04-14 | GSE198001 | GEO
Project description:Important regulation occurs at the level of transcription in Plasmodium falciparum and growing evidence suggests that these apicomplexan parasites have complex regulatory networks. Recent studies implicate long noncoding RNAs (lncRNAs) as transcriptional regulators in P. falciparum and as part of these regulatory networks. However, due to limited research and the lack of necessary experimental tools, our understanding of the role of lncRNAs in P. falciparum remains largely unelucidated. In this study, we improve the annotation of lncRNAs in order to provide researchers with a tool that will facilitate the study of P. falciparum lncRNAs in vitro.
2022-06-10 | E-MTAB-11766 | biostudies-arrayexpress
Project description:Benzoxaboroles (BoBs) feature a boron-heterocyclic core and are an important recent innovation in the development of drugs against a range of pathogens and other pathologies. A broad spectrum of pharmacology is associated with chemically diverse BoB derivatives and includes multiple modes-of-action and targets. However, a consensus MoA for BoBs targeting evolutionarily diverse protozoan pathogens has emerged with the identification of CPSF3/CPSF73 in the CPSF complex in both apicomplexan and kinetoplastida parasites. We have detected a functional connection between protein sumoylation and the BoB boron-heterocyclic scaffold using comprehensive genetic screens in Trypanosoma brucei. Strikingly, as part of this sumoylation response, members of the CPSF complex are specifically and rapidly destabilised in a SUMO and proteosome-dependent manner. Here we deposit RNAseq data quantifying the effects of the aminomethyl-benzoxaborole AN3057 exposure on the transcriptome landscape in T. brucei. Specifically, T. brucei bloodstream-form cells in logarithmic growth phase were treated with 400 nm AN3057 (3 × EC50 determined after 24h) for 20 min (T20) and 60 min (T60), respectively. Nontreated control cells were prepared in parallel. All samples were in 2 biological replicates.
2024-03-08 | E-MTAB-13843 | biostudies-arrayexpress
Project description:In this study we have investigated PfAP2-HC (PF3D7_1456000), a protein that was identified by co-immunoprecipitation with PfHP1 coupled with liquid chromatography-tandem mass spectrometry. PfAP2-HC belongs to the ApiAP2 family, the main transcription factor family in Apicomplexan parasites. We have confirmed that AP2-HC colocalises with HP1 with the use of immunofluorescence assays and chromatin immunoprecipitation-sequencing. We show that PfAP2-HC is not required for heterochromatin maintenance and inheritance with the use of PfAP2-HC Kock out and knockdown. We show with transcriptome-wide microarray time course analysis that PfAP2-HC does not act as a transcription factor in blood stage parasites. We demonstrate that the AP2 domain is dispensable for heterochromatin targeting by introducing a premature stop codon before the AP2 domain. We show that PfAP2-HC binding to heterochromatin dependents on PfHP1. and PfAP2-HC is likely not involved in de novo heterochromatin formation
2020-11-04 | GSE154840 | GEO