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PROVIDER: PRJNA682758 | ENA |
REPOSITORIES: ENA
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Project description:Background and aims The endophytic diazotrophic strain CBAmC of Nitrospirillum amazonense has been reported as a plant growth promoter of sugarcane variety RB867515 when grown under field conditions. The present work aimed to assess the influence of apoplast fluid from RB867515 on the transcriptomic and proteomic profiles of CBAmC cultured in vitro. Methods RNA-Seq in Ion Proton™ and ESI-LC-MS/MS peptide analysis were used to evaluate the transcriptomic and proteomic profiles, respectively, of CBAmC exposed for 2 h to the sugarcane apoplast fluid. Results The bacterial transcriptomic and proteomic profiles were well correlated. The overall response of CBAmC to the apoplast fluid included overexpression of defense systems against reactive oxygen species (ROS) and osmotic stress, RND efflux pumps for toxic compounds, Sec and Tat secretory systems, and assimilative metabolism of iron. In contrast, active transporters of organic compounds, chemotaxis system and flagellum structure were underexpressed. Conclusions The bacterial metabolic pathways / functions activated in response to the sugarcane apoplast fluid are most likely related to its adaptation to the peculiar characteristics of the fluid. The activation of some of those functions could be determinant for its adaptation to the sugarcane apoplastic niche, and perhaps be involved in the previously observed effect of promoting plant growth. SUBMITTER_CITATION: Terra, L.A., de Soares, C.P., Meneses, C.H.S.G. et al. Plant Soil (2019). Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid.
2019-04-26 | GSE130321 | GEO
Project description:To explore the molecular mechanism of low-K tolerance in sugarcane, we have employed whole genome microarray expression profiling to identify sugarcane genes in response to low-K stress. seeldings were transplanted to low-K hydroponic (containing 0.1 mmol.L-1 K+) and the roots were collected at 0 (CK), 8, 24 and 72 h after exposure to low-K condition. The expressions of genes in sugarcane roots were detected by microarray analysis. Totally 1545 genes at 8 h, 1053 genes at 24 h and 3155 at 72 h differentially expressed under low-K stress, when the 2-fold change was adopted as the threshold for determining differentially expressed genes. Among these genes, a certain amount of transcription factors, transporters, kinases, oxidative stress-related genes and genes in Ca+ and ethylene signaling pathway were detected to differentially express. Seeldings were treated with low-K hydroponic (containing 0.1 mmol.L-1 K+) and after 0 (CK), 8, 24 and 72 h exposure to low -K stress, the roots of sugarcane were collected. Four independent experiments were performed using roots collected at different time points
2014-10-02 | E-GEOD-61935 | biostudies-arrayexpress
Project description:In order to increase our understanding on the epigenetic regulation in response to abiotic stresses in plants, sRNA regulation in sugarcane plants submitted to drought stress was analyzed. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. An enrichment of 22-nt sRNA species was observed in leaf libraries. The pool of sRNA selected allowed the analysis of different sRNA classes (miRNA and siRNA). Twenty eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profile of eight miRNA was verified in leaf samples by stem-loop qRT-PCR assay. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. 22-nt miRNA triggered siRNA-candidates production by cleavage of their targets in response to drought stress. Some genes of sRNA biogenesis were down-regulated in tolerant genotypes and up-regulated in sensitive in response to drought stress. Our analysis contributes to increase the knowledge on the roles of sRNA in epigenetic-regulatory pathways in sugarcane submitted to drought stress. Screenning of sRNA transcriptome of sugarcane plants under drougth stress
2012-11-24 | E-GEOD-42483 | biostudies-arrayexpress
Project description:Recirculation systems (RAS), which reduce water consumption and improve pathogen control are increasingly used in Atlantic salmon aquaculture. Performance and adaptation of fish to new farming environment is actively investigated. Here, responses to crowding stress were compared in salmon reared in two systems (RAS with brackish water and flowthrough with full salinity water) at low and high density.
2021-04-27 | GSE173229 | GEO
Project description:<p>Chicken farming is critical for future food security, yet there is still a need for improving animal welfare and optimised feeding strategies. HoloFood will explore the interaction and impact of the microbiome together with the host (animal) in response to different feeding strategies already implemented commercially. Chicken farming has recently made huge efforts towards healthy, natural food production without using chemicals or antibiotics in food systems. As such natural alternatives, such as prebiotic and probiotic additives to chicken feed, have been a huge improvement. Nonetheless, the full impact of these natural alternatives and the variation in chicken growth is still poorly understood. HoloFood aims to investigate the effect and interplay between the host (chicken) - microbiome in response to different dietary additives.</p><p><br></p><p><strong>HoloFood Trial 1/2/3</strong> is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS6988' rel='noopener noreferrer' target='_blank'><strong>MTBLS6988</strong></a>.</p><p><strong>HoloFood Trial A - seaweed-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4381' rel='noopener noreferrer' target='_blank'><strong>MTBLS4381</strong></a>.</p><p><strong>HoloFood Trial B - blue mussel-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4382' rel='noopener noreferrer' target='_blank'><strong>MTBLS4382</strong></a>.</p><p><strong>HoloFood Trial C - blue mussel ensilage-dose response</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS4384' rel='noopener noreferrer' target='_blank'><strong>MTBLS4384</strong></a>.</p><p><strong>HoloFood Trial D - fermented seaweed open water-dose</strong> response is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS6733' rel='noopener noreferrer' target='_blank'><strong>MTBLS6733</strong></a>.</p><p><br></p><p><strong>Linked cross omic data sets:</strong></p><p>Nucleic acid data associated with this study are available in the European Nucleotide Archive (ENA): accession number <a href='https://www.ebi.ac.uk/ena/browser/view/PRJEB43192' rel='noopener noreferrer' target='_blank'>PRJEB43192</a>.</p><p>Metagenomic data associated with this study are available from MGnify under the Super Study '<a href='https://www.ebi.ac.uk/metagenomics/super-studies/holofood' rel='noopener noreferrer' target='_blank'>holofood</a>'.</p>
2023-06-13 | MTBLS6988 | MetaboLights