Project description:Bacterial and fungal infections induce a potent immune response in Drosophila melanogaster, but it is unclear whether viral infections induce a similar immune response. Using microarrays, we examined the changes in gene expression in Drosophila that occur in response to infection with the sigma virus, a negative-stranded RNA virus (Rhabdoviridae) that occurs in wild populations of D. melanogaster. We detected many changes in gene expression in infected flies, but found no evidence for the activation of the Toll, IMD or Jak-STAT pathways, which control immune responses against other pathogens. We identified a number of functional categories of genes, including serine proteases, ribosomal proteins and chorion proteins that were overrepresented among the differentially expressed genes. We also found that the sigma virus alters the expression of many more genes in males than in females. In contrast to previous results, our data suggest that either Drosophila do not mount an immune response against the sigma virus, or that the immune response is not controlled by known immune pathways. The genes which we identified as differentially expressed after infection are promising candidates for controlling the host’s response to the sigma virus.
2009-09-11 | GSE17377 | GEO
Project description:Drosophila melanogaster infected with Invertebrate iridescent virus 6 (IIV6) Small RNAs deep sequencing
Project description:Wolbachia is a maternally transmitted bacterium that manipulates arthropod and nematode biology in myriad ways. The Wolbachia strain colonizing Drosophila melanogaster creates sperm-egg incompatibilities and protects its host against RNA viruses, making it a promising tool for vector control. Despite successful trials using Wolbachia-transfected mosquitoes for Dengue control, knowledge of how Wolbachia and viruses jointly affect insect biology remains limited. Using the Drosophila melanogaster model, transcriptomics and gene expression network analyses revealed pathways with altered expression and splicing due to Wolbachia colonization and virus infection. Included are metabolic pathways previously unknown to be important for Wolbachia-host interactions. Additionally, Wolbachia-colonized flies exhibit a dampened transcriptomic response to virus infection, consistent with early blocking of virus replication. Finally, using Drosophila genetics, we show Wolbachia and expression of nucleotide metabolism genes have interactive effects on virus replication. Understanding the mechanisms of pathogen blocking will contribute to the effective development of Wolbachia-mediated vector control programs.
Project description:We infected Drosophila S2 cells (invitrogen) with Drosophila C virus (DCV) (Multiplicity of Infection = 10), and harvested samples for further analysis at 8 and 24 hours post-infection.
Project description:RNAseq analysis of human lung tissue explants infected with Influenza B virus Results: Differentially expressed genes after infection Project: Hulu_FluB_2022
Project description:Female mouse was infected by zika virus, then subject to 5hmC capture and RNAseq, by comparing PBS treated mouse and zika infected mouse, we identified zika affected gene that regulated by 5hmC change
Project description:Female mouse was infected by zika virus, then subject to 5hmC capture and RNAseq, by comparing PBS treated mouse and zika infected mouse, we identified zika affected gene that regulated by 5hmC change
