Project description:Neuroblastoma is a pediatric tumor of the developing sympathetic nervous system. We investigate developmental origins of neuroblastoma, the role of oncogenic MYCN in blocking normal differentiation and evaluate therapeutic interventions to overcome differentiation blocks. Here, we provide expression profiles of SK-N-BE(2)C cells upon treatment with ATRA or solvent control.
Project description:Neuroblastoma is a pediatric tumor of the developing sympathetic nervous system. We investigate the developmental origin of neuroblastoma, the role of oncogenic MYCN in blocking normal differentiation and evaluate therapeutic interventions to overcome differentiation blocks. Here, we provide single cell expression profiles (scRNA-seq MARS-seq) of SK-N-AS cells (n=603).
Project description:Following treatment with the de-methylating agent 5-aza-deoxycytidine (DAC), the gene expression profiles of neuroblastoma cell lines Kelly, SK-N-AS and NGP were analysed in order to examine the relationship between transcriptional re-activation and promoter region DNA methylation.<br>In addition, the neuroblastoma cell line SK-N-BE was treated with all-trans-retinoic acid (ATRA) in order to examine the impact this differentiation agent has on DNA methylation status.
Project description:Neuroblastoma is a pediatric tumor of the developing sympathetic nervous system. We investigate developmental origins of neuroblastoma, the role of oncogenic MYCN in blocking normal differentiation and evaluate therapeutic interventions to overcome differentiation blocks. Here, we provide single cell expression profiles of IMR5/75-shMYCN cells upon knockdown of MYCN and control.
Project description:Analysis of differential gene expression in response to treatment of neuroblastoma cell lines (MYCN-amplified SK-N-BE(2)-C and non-amplified SKNAS) with retinoic acid and verlindamycin (a novel polyamine analogue and LSD1 inhibitor). This compound combination was found efficient in inducing differentiation in neuroblastoma cell lines and the goal of this microarray experiment was to analyse which genes and pathways were altered upon this treatment.
Project description:In the absence of recurrent gene mutations, evidence accumulates that epigenetic deregulation plays a prominent role in neuroblastoma biology. Here we provide single cell expression profiles (scRNA-seq MARS-seq) of SK-N-SH cells (n=1176)