Project description:Parascedosporium putredinis NO1 was grown for 4 days on six lignocellulosic substrates: Kraft Lignin (LI), Sugar Cane Bagasse (SC), Rice Straw (RS), Wheat Straw (WS), Wheat Bran (WB), and Empty Fruit Bunches from Palm Oil (EF). Proteins were harvested from the culture supernatant and from the insoluble fraction using a biotin-labelling approach to target the proteins bound to the lignocellulosic substrates.
Project description:To determine the mechanisms of fleshy fruit abscission of the monocot oil palm (Elaeis guineensis Jacq.) compared with other abscission systems, we performed multi-scale comparative transcriptome analyses on fruit targeting the developing primary AZ and adjacent tissues. Combining between-tissue developmental comparisons with exogenous ethylene treatments, and naturally occurring abscission in the field, RNAseq analysis revealed a robust core set of 168 genes with differentially regulated expression, spatially associated with the ripe fruit AZ, and temporally restricted to the abscission timing. The expression of a set of candidate genes was validated by qRT-PCR in the fruit AZ of a natural oil palm variant with blocked fruit abscission, which provides evidence for their functions during abscission. Our results substantiate the conservation of gene function between dicot dry fruit dehiscence and monocot fleshy fruit abscission. The study also revealed major metabolic transitions occur in the AZ during abscission, including key senescence marker genes and transcriptional regulators, in addition to genes involved in nutrient recycling and reallocation, alternative routes for energy supply and adaptation to oxidative stress. The study provides the first reference transcriptome of a monocot fleshy fruit abscission zone and provides insight into the mechanisms underlying abscission by identifying key genes with functional roles and processes, including metabolic transitions, cell wall modifications, signalling, stress adaptations and transcriptional regulation, that occur during ripe fruit abscission of the monocot oil palm. The transcriptome data comprises an original reference and resource useful towards understanding the evolutionary basis of this fundamental plant process.
Project description:Sodium deoxycholate was evaluated as alternative protein solubilization buffer to a more commonly used urea/thiourea buffer. Sodium deoxycholate was found to have similar efficiency as of urea/thiourea buffer in solubilizing proteins from oil palm fruit mesocarps.
Project description:C57Bl/6J mice were put on a low fat diet (10 kcal% palm oil) or high fat diet (60 kcal% palm oil), combined with a low dietary Mg2+ (0.03 w/w% or 0.21 w/w%) for a period of 17 weeks. White adipose tissue was harvested and total RNA isolated using TRIzol, of which 5 samples per group were sent to BGI-China for RNA-Sequencing.
Project description:Proteome profiles of oil palm mesocarp from different stages of fruit development were analysed using two-dimensional gel electrophoresis (2DE) and the significantly changed protein were identified using by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF/TOF) and functionally classified using ontology analysis.
Project description:Oil palm breeding and seed development have been hindered due to the male parent's incapacity to produce male inflorescence as a source of pollen under normal conditions. On the other hand, a young oil palm plantation has a low pollination rate due to a lack of male flowers. These are the common problem of sex ratio in the oil palm industry. Nevertheless, the regulation of sex ratio in oil palm plants is a complex mechanism and remains an open question until now. Researchers have previously used complete defoliation to induce male inflorescences, but the biological and molecular mechanisms underlying this morphological change have yet to be discovered. Here, we present an RNA-seq dataset from three early stages of an oil palm inflorescence under normal conditions and complete defoliation stress. This transcriptomic dataset is a valuable resource to improve our understanding of sex determination mechanisms in oil palm inflorescence.
Project description:The coconut tree (Cocos nucifera L.) is an ancient palm species that since early times had been integrally exploited due to their many benefits to the habitants of the tropical and subtropical areas (Niral and Jerard, 2018). The coconut together with the oil palm (Elaeis guineensis) and date palm (Phoenix dactylifera L.) belongs to the Arecaceae family and represent a natural source of oils and carbohydrates both, highly demanded in food and pharmaceutical industries. The coconut shows high morphological variations, but grouping normally in two groups, according to their morphology and growth habits, the “Tall” and the “Dwarf” varieties. Frequently, hybrids are considered as a third variety resulting from cross pollination between Tall and Dwarfs. Tall varieties begin to flowering at 5-7 years after planting, and continue emitting inflorescences and fruits up to 80 to 100 years. When adults, tall palms reach heights between 20-30 m; producing fruits from medium to large in size with abundant solid endosperm and high oil content. In case of dwarf palm varieties, they start flowering and fruiting at 3-4 years after planting, and continue producing by almost 50 years. As adults, their height is 8-10 m, producing fruits from small to medium size with moderate amounts of solid endosperm and less oil content than tall varieties. The solid endosperm, also named as “coconut meat” and when it is dry “copra”, is the source of medium chain saturated fatty acids (MCSFA), e.g. lauric acid (C12:0), myristic acid (C14:0) and palmitic acid (C16:0), among others, and their content of total fatty acids is higher in Tall than in Dwarf varieties. Coconut Oil contains high levels of lauric acid and exhibit characteristics as increased oxidative stability, low melting points and formation of stable emulsions, all them highly appreciated in the food and chemical industries (Kumar, 2011; Reynolds et al., 2019). Moreover, due to its high content of antioxidants such as tocopherol and beta-carotene, coconut oil exhibit various health benefits, such as antibacterial, antiviral, and cardiovascular protection. Coconut fruit growth and ripening is intrinsically related with development of the different seed tissues, i.e., endosperms, embryo and pericarp. The endosperms of the coconut fruit did not accumulate synchronically with fruit maturation.
Project description:Oil palm (Elaeis guineensis Jacq.) is one of the most important oil-producing crops in the world. However, the demand for oil from this crop will increase in the future. A comparative gene expression profile of the oil palm leaves was needed in order to understand the key factors that influence the oil production. Here, we reported an RNA-seq dataset from three different oil yields and three different genetic populations of oil palm. All raw sequencing reads were obtained from an Illumina NextSeq 500 platform. We also provide a list of the genes and their expression levels resulting from the RNA-sequencing. This transcriptomic dataset will provide a valuable resource for increasing oil yield.
Project description:H3K4me3 profiling of OSCC secondary (2ary) recipient primary tumors (PTs) following in vivo exposure to control (CTRL) or palm oil-enriched (PALM) diet in primary recipient mice