Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Regulatory neutrophil transcriptional profiling

ABSTRACT: Regulatory neutrophil transcriptional profiling

PROVIDER: PRJNA700601 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Regulatory neutrophil transcriptional profiling

Project description:We report that pharmacologically-induced autoantigen-specific T-regulatory type 1 (TR1) cells and TR1 cell-induced B-regulatory (Breg) cells recruit neutrophils into the liver and program their transcriptome to generate regulatory neutrophils

2021-04-05 | GSE166361 | GEO

Transcriptional checkpoints control Neutrophil-driven inflammation

Project description:Transcriptional checkpoints control Neutrophil-driven inflammation

| PRJNA679311 | ENA

Time-dependent transcriptional changes in neutrophil phenotype

Project description:Time-dependent transcriptional changes in neutrophil phenotype

| PRJNA397395 | ENA

Transcriptional profiling of Regulatory T (Treg) cells in obesity

Project description:Transcriptional profiling of Regulatory T (Treg) cells in obesity

| PRJNA731160 | ENA

Transcriptional checkpoints control Neutrophil-driven inflammation

Project description:This dataset combines transcriptional and chromatin analysis of neutrophils from the air pouch mouse model, an in vivo model of acute inflammation, with transcriptional analysis of Hoxb8 neutrophils, an in vitro model of neutrophil development.

2021-05-09 | GSE161765 | GEO

Transcriptional profiling

Project description:Transcriptional profiling

| PRJNA714917 | ENA

Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection

Project description:The full neutrophil heterogeneity and differentiation landscape remains incompletely characterized. Here we profiled more than 25,000 mouse differentiating and mature neutrophils using single-cell RNA sequencing to provide a comprehensive transcriptional landscape of neutrophil maturation, function, and fate decision in their steady state and during bacterial infection. Eight neutrophil populations (including the GMP population) were defined by distinct molecular signatures, including a new circulating mature neutrophil population highly expressing interferon-stimulated genes. The three mature peripheral blood neutrophil subsets arise from distinct maturing bone marrow neutrophil subsets, a novel mechanism that highlights the complex and precise regulation of neutrophil production. Neutrophil heterogeneity and differentiation are driven by both known and uncharacterized transcription factors. Neutrophils gradually acquire microbicidal capability as cells traverse the transcriptional landscape, representing an evolved mechanism for fine-tuned regulation of an effective but balanced neutrophil response. Bacterial infection reprograms the genetic architecture of neutrophil population, alters dynamic transition between each subpopulation, and primes neutrophils for augmented functionality without affecting overall heterogeneity. Bacterial infection-induced emergency granulopoiesis is mediated by augmented proliferation of early-stage neutrophil progenitors and accelerated post-mitotic maturation. In summary, single-cell transcriptomics enabled the reconstruction of neutrophil differentiation and maturation trajectories and uncovered neutrophil subpopulations, gene pathways, and regulators of neutrophil function and fate decisions. These data establish a reference model and general framework for studying neutrophil-related disease mechanisms, biomarkers, and therapeutic targets at single-cell resolution.

2020-05-27 | GSE137539 | GEO

Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane

Project description:In the present work neutrophil subcellular fractionation by density gradient centrifugation was conducted in order to perform a quantitative proteomic profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane

2017-06-23 | PXD005559 | Pride

Transcriptional Regulation of HL60 Neutrophil Differentiation: Promyelocyte trajectory vs. Neutrophil trajectory

Project description:Using the HL60 multipotent promyelocytic leukemia cell line, we performed experiments that lead to two different cell fate attractors by treatments of varying dosage and duration of the differentiation agent all-trans-retinoic acid (ATRA). The dosage and duration combinations corresponding to the two treatments were chosen by means of of cytometric measurements of CD11b, a well-known early differentiation marker, such that the two populations are poised at the same stage of differentiation. Using the selected treatment combinations, one population proceeds toward the terminally differentiated neutrophil attractor while the other population reverts back toward the undifferentiated promyelocytic attractor. We monitored the gene expression changes in the two populations after their respective treatments over a period of five days and identified a set of genes that diverged in their expression; a subset of which promotes neutrophil differentiation while the other represses cell cycle progression. By employing promoter based transcription factor binding site analysis, we found enrichment of transcription factors functionally linked to tumor progression, cell cycle, and development. Keywords: Time course, dose response Untreated HL60 cells are hybrized on the channel 1 with Cy3, ATRA-Treated HL60 cells are hybridized on channel 2 with Cy5. HK60 cells were treated with high dosage/short duration and low dosage/long duration to achieve 55% of CD11b+ cells. These positive cells were then isolated and culture in fresh media and total RNA were isolated for comparison. Cells treated with high dosage/short duration treatment proceed toward the neutrophil attractor while cells treated with the low dosage/long duration treatment revert back toward the promyelocyte attractor.

2008-11-19 | E-GEOD-13673 | biostudies-arrayexpress

Gene expression analysis of predestined heterogeneity in neutrophil progenitor clones (ER-HoxB8 conditionally immortalized)

Project description:Transcriptional profiling of clonal neutrophil progenitor populations. Conditionally immortalized CD45.1 naive paired granulocyte-monocyte progenitors (GMPs) and their mature neutrophil counterpart were profiled in an in vitro cell system. Clones demonstrated an overall similar transcriptional profile within the GMP or neutrophil states, however, individual clones had several differentially expressed genes (DEGs) between each other at both the progenitor and mature cell stages.

2022-09-27 | GSE188679 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data