Project description:In order to explore the potential role of escape of late apoptosis in metastasis, we induced apoptosis and subsequently inhibited the death process in a primary culture of colon cancer cells. Here we wanted to explore the transcriptome of such "undead" cells 7 days after apoptosis induction.
Project description:In order to explore the potential role of escape of late apoptosis in metastasis, we induced apoptosis and subsequently inhibited the death process in a primary culture of colon cancer cells. Here we wanted to explore the transcriptome of cells treated with the conditioned medium of these surviving cells. Thes cells have been treated for 48h with conditionned media previous RNA extraction and RNAseq.
Project description:Showing the anti cancer activity of Cerulenin on colon cancer cell lines. The study shows the mechanismm of apoptosis induction as a result of cerulenin treatment to colon cancer cell lines.
Project description:The objective of this study was to gain insights into the biological basis of colon cancer progression by characterizing gene expression differences between normal colon epithelium, corresponding colorectal primary tumors and metastases. We found a close similarity in gene expression patterns between primary tumors and metastases, indicating a correlation between gene expression and morphological characteristics. PRDX4 was identified as highly expressed both in primary colon tumors and metastases, and selected for further characterization. Our study revealed that Prdx4 (PrxIV, AOE372) shows functional similarities to other Prx family members by negatively effecting apoptosis induction in tumor cells. In addition, our studies link Prdx4 with Hif-1M-NM-1, a key regulatory factor of angiogenesis. Targeting Prdx4 may be an attractive approach in cancer therapy, as its inhibition is expected to lead to induction of apoptosis and blockade of Hif-1M-NM-1-mediated tumor angiogenesis. mRNA expression profiling of normal colon epithelium (5), primary colon tumors (12 with 1 replicate) and either lymph node metastases (9 with 2 replicates) or liver metastases (2) and cell lines (4) from twelve colon cancer patients.
Project description:The objective of this study was to gain insights into the biological basis of colon cancer progression by characterizing gene expression differences between normal colon epithelium, corresponding colorectal primary tumors and metastases. We found a close similarity in gene expression patterns between primary tumors and metastases, indicating a correlation between gene expression and morphological characteristics. PRDX4 was identified as highly expressed both in primary colon tumors and metastases, and selected for further characterization. Our study revealed that Prdx4 (PrxIV, AOE372) shows functional similarities to other Prx family members by negatively effecting apoptosis induction in tumor cells. In addition, our studies link Prdx4 with Hif-1α, a key regulatory factor of angiogenesis. Targeting Prdx4 may be an attractive approach in cancer therapy, as its inhibition is expected to lead to induction of apoptosis and blockade of Hif-1α-mediated tumor angiogenesis.
Project description:Inhibition of Akt Signaling in PIK3R2 Overexpressing Colon Cancer Stem Cells Reduces Tumor Growth due Apoptosis and Decreased Mucin Production