Project description:Characterization of the impact of TT2 or MYB5 overexpression on gene expression. Col 0 plants were compared to 35S::TT2 and 35S::MYB5 overexpressors in control conditions
Project description:Purpose: To understand the molecular mechanism between PNET2 and NTLs, we generated 35S:NTL-SRDX in pnet2_ab mutants to suppress NTL gene expression and performed the whole genome transcriptome analysis on 4-week-old plants of WT, pnet2_ab, 35S:NTL6-SRDX pnet2_ab, 35S:NTL9-SRDX pnet2_ab, and 35S:NTL12-SRDX pnet2_ab. Conclusions: PNET2 regulates diverse responses by associating with a family of membrane-bound transcriptional factors.
Project description:ra07-05_myb82 - functional characterization of vvmyb82 - Functional characterization of the transcription factor VvMYB82 which is a regulator of the different phenolic compound biosynthesis. - To compare wt with a surexpressor of VvMYB82 (Line A311 = 35s::VvMYB) and wt with a loss of function mutant AtMYB82
Project description:Transcriptomic analysis of a dne1-1 mutant, two overexpressors of DNE1 (P35S-GFP::DNE1), an overexpressor of DNE1_D153N (35S:GFP-DNE1_D153N), and vcs-6. Biological triplicates of 14 days old seedlings were analyzed.
Project description:ra07-05_myb82 - functional characterization of vvmyb82 - Functional characterization of the transcription factor VvMYB82 which is a regulator of the different phenolic compound biosynthesis. - To compare wt with a surexpressor of VvMYB82 (Line A311 = 35s::VvMYB) and wt with a loss of function mutant AtMYB82 2 dye-swap - wt vs mutant comparison
Project description:To identify the genes regulated by Arabidopsis ERF19. 35S::ERF19 transgenic Arabidopsis was created, them we employ whole genome microarray expression profiling as a discovery platform to identify genes with the potential to regulae by ERF19. The RNA was isopated from the whole flower of control and 35S::ERF19 transgenic Arabidopsis.
Project description:Arabidopsis CLE41 regulates vascular organisation, cell division and xylem differentiation. 35S::CLE41 lines are characterised by an increase in the number of undifferentiated cells in vascular bundles and by radial patterning defects. To identify further genetic components involved in controlling vascular development, we compared the transcriptomes of one 35S::CLE41 line with that of wild type counterparts using microarray analysis. Inflorescence stem tissue was compared in 5 weeks old plants 1 cm above the rosette leaves where vascular bundles of 35S::CLE41 plants contained an average of 100.7 ± 9.1 undifferentiated cells compared to 59.5 ± 5.5 in wild type counterparts. Genes with a putative role in vascular development demonstrate changes in gene expression between the two genotypes tested.