Project description:In this study, the relative effect of consuming five different commercial feeds (Feed B-F) and and frozen fish (control; Feed A) for two months on our selected juvenile Asian seabass (77.3g ± 22.4g) were investigated. The growth performance, the biochemical analysis of their flesh, the histology of their midgut and the transcriptome of their midgut and liver were compared to each other and controls. Our customised agilent microarray platform (GPL17855), were used to investigate the liver transcriptomic expression of the different Groups fed with different Feeds. No differential expression were detected between commerical Feeds (B, C, E and F). A total of 397 differentially expressed transcripts (Foldchange cutoff: -1.5⤠or â¥1.5, Pvalue with FDR (<0.05) were detected between Feed D and control Feed A. Total: 24 samples (4 biological samples per Group; 6 Different Groups in total). Groups (A-F) were fed with Feeds (A-F) respectively for 61 days.
Project description:In this study, the relative effect of consuming five different commercial feeds (Feed B-F) and and frozen fish (control; Feed A) for two months on our selected juvenile Asian seabass (77.3g ± 22.4g) were investigated. The growth performance, the biochemical analysis of their flesh, the histology of their midgut and the transcriptome of their midgut and liver were compared to each other and controls. Our customised agilent microarray platform (GPL17855), were used to investigate the liver transcriptomic expression of the different Groups fed with different Feeds. No differential expression were detected between commerical Feeds (B, C, E and F). A total of 397 differentially expressed transcripts (Foldchange cutoff: -1.5≤ or ≥1.5, Pvalue with FDR (<0.05) were detected between Feed D and control Feed A.
Project description:The peanut worm (Sipunculus nudus) is an economically important fishery resource in China. To determine how dietary carbohydrate and protein levels affects the growth performance of juvenile S. nudus and identify the mechanisms underlying observedpatterns, five isoenergetic and isolipidic diets with different levels of carbohydrate and protein were formulated and fed to juvenile S. nudus; the experimental groups were referred to as EG1, EG2, EG3, EG4, and EG5, respectively. After 90 days of feeding, S. nudus had significantly lower survival rates when fed D5 compared with other diets (P < 0.05), and the highest survival rate was observed in EG2 individuals. The weight gain rate and specific growth rate were significantly higher in EG2 compared with the other groups (P < 0.05). Metabolomic profiling using LC–MS revealed 83 significantly differential metabolites (POS: 59; NEG: 24), which were identified via an in-house MS2 database. Pathway analysis indicated that the significantly different metabolites were involved in 22 metabolic pathways (POS: 9; NEG: 13), including tyrosine, phenylalanine, and tryptophan biosynthesis; phenylalanine metabolism; D-glutamate and D-glutamine metabolism; proline and arginine metabolism; aspartate, alanine, and glutamate metabolism; and aminoacyl-tRNA biosynthesis. These analyses implied that the biosynthetic capabilities of juvenile S. nudus were greater in the EG2. The results of this research enhance our understanding of the effects of dietary carbohydrate and protein levels on the growth performance of juvenile S. nudus.