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Transcriptomic analysis of E9.5 mouse embryos

ABSTRACT: Transcriptomic analysis of E9.5 mouse embryos

PROVIDER: PRJNA727827 | ENA |

REPOSITORIES: ENA

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Transcriptomic profiling of E9.5 mouse embryos lacking the lipoprotein receptor SR-B1

Project description:The high-density lipoprotein receptor SR-B1 mediates cellular uptake of several lipid species, including cholesterol and vitamin E. During early development, SR-B1 is located in the maternal-fetal interface, where it facilitates vitamin E transport towards the embryo. Consequently, embryos lacking SR-B1 are vitamin E-deficient, and around half of them fail to close the neural tube and show neural tube defects (NTD). Here, we studied the transcriptomic profile of mouse embryos lacking SR-B1 to identify the molecular determinants of this phenotypic difference. We used RNA-Seq to analyze the expression of mRNA globally in E9.5 wild-type embryos and embryos lacking SR-B1 with or without NTD, in order to compare expression profiles in those groups and to identify putative genes driving phenotypic differences.

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Transcriptomic profiling of E9.5 mouse embryos lacking the lipoprotein receptor SR-B1

Project description:Transcriptomic profiling of E9.5 mouse embryos lacking the lipoprotein receptor SR-B1

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Genome wide RNA-seq analysis of E9.5 Smchd1 wild type and mutant embryos

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Genome wide RNA-seq analysis of E9.5 Smchd1 wild type and mutant embryos

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DNA methylation analysis by MeDIP-on-chip in mouse E3.5 blastocysts, E6.5 epiblasts and E9.5 whole embryos.

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