Project description:We report the application of MeRIP-seq to map m6A peaks in wild type and METTL5 KO HeLa cells to investigate targets of the m6A methyltransferase METTL5.
Project description:HCC cell line, Huh-7 cells and HCC-LM3 cells, was transfected with METTL5 sgRNA to knockout METTL5 expression., and check the downstream mRNA changes.
Project description:We report the application of next-generation sequencing to analyze the transcriptomes of brains and livers of WT and METTL5 KO mice to understand the role(s) of METTL5 in these organs.
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived embryonic stem cells (ESCs) transcriptome. Methods: mESCs mRNA profiles of wild-type (WT) and methyltransferase like 5 knockout (Mettl5−/−) mice were generated by using oligo(dT)-attached magnetic beads enrichemnt. The sequence reads that passed twice quality filters were analyzed at the transcript isoform level with Bowtie2 for quantitative gene analysis and with DEGseq for differentially expressed genes analysis. Results: 15 samples were tested by using the BGISEq-500 platform, and each sample produced 21.56 M data on average. The average ratio of samples to genomes (mm10) was over 94%; A total of 18762 genes were detected.