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Direct RNA ONT sequencing of pine roots under ammonium nutrition

ABSTRACT: Direct RNA ONT sequencing of pine roots under ammonium nutrition

PROVIDER: PRJNA731860 | ENA |

REPOSITORIES: ENA

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Direct RNA ONT sequencing of pine roots under ammonium nutrition

Project description:Epitranscriptomics modifications constitute a gene expression checkpoint in all living organism including plants. Considering the relevance of nitrogen nutrition and metabolism for the correct plant growth and development, it can be hypothesized that epitranscriptome changes must regulate every biological process in plants including nitrogen nutrition. In the present work, the epritranscriptomics changes in maritime pine roots caused by ammonium nutrition have been monitored through direct RNA sequencing using Oxford Nanopore Technology. The main transcriptome responses to ammonium nutrition affected to transcripts involved in nitrogen and carbon metabolisms, defense response, hormone synthesis and signaling, and translation. Additionally to a global detection of epitranscriptomics marks, the m6A deposition and its dynamics have been identified, which seems to be important regulators of translation when compared with the proteomic profiles of the same samples. In this sense, the obtained results suggest that protein translation is finely regulated through the epitranscriptomics marks maybe through changes in mRNA polyA length, transcript amount and ribosome protein composition. The multiomics results in the present study suggest that the epitranscriptome must modulate the responses to development and environmental changes, including ammonium nutrition, through buffering, filtering and focusing the final products of the gene expression.

2021-05-22 | GSE174830 | GEO

miRNA expression of Pinus pinaster roots under ammonium nutrition

Project description:In the present study, the miRNA expression changes in the maritime pine roots from seedlings of one-month old under different levels of ammonium nutrition was analyzed.

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Proteomic profile of Pinus pinaster roots under ammonium nutrition

Project description:Ammonium nutrition was studied at short times (24 hours) in roots from one month-old seedlings of maritime pine. Control seedlings were irrigated with 80 mL of water (C) and the experimental seedlings with 80 mL of 3 mM NH4Cl. Root samples were collected at 24 hours post-irrigation and immediately frozen in liquid N. This experiment was carried out three independent times.

2023-03-11 | PXD025331 | Pride

miRNA expression of Pinus pinaster roots under ammonium nutrition

Project description:miRNA expression of Pinus pinaster roots under ammonium nutrition

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N6- methyladenosine (m6A) detection in ammonium treated seedlings through RNA immunoprecipitation

Project description:Epitranscriptomics modifications constitute a gene expression checkpoint in all living organism including plants. Considering the relevance of nitrogen nutrition and metabolism for the correct plant growth and development, it can be hypothesized that epitranscriptome changes must regulate every biological process in plants including nitrogen nutrition. In the present work, the epritranscriptomics changes in maritime pine roots caused by ammonium nutrition have been monitored through RIP-seq. The main transcriptome responses to ammonium nutrition affected to transcripts involved in nitrogen and carbon metabolisms, defense response, hormone synthesis and signaling, and translation. The m6A deposition and its dynamics have been identified, which seems to be important regulators of translation when compared with the proteomic profiles of the same samples. The present study suggest that the epitranscriptome must modulate the responses to development and environmental changes, including ammonium nutrition, through buffering, filtering and focusing the final products of the gene expression.

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Interaction between CO2 levels and nitrogen nutrition in Pinus pinaster

Project description:The main objective of the present work is the transcriptomic analysis of the interaction between nitrogen nutrition and CO2 levels in maritime pine. For this purpose, seedlings were fertilized with nitrate or ammonium and grown in two different CO2 levels: normal (400 ppm) and high (720ppm).

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Gene expression in tissues of root tips from Pinus pinaster seedlings under ammonium nutrition

Project description:In the present study, the gene expression changes in four different tissues of root tips from maritime pine were analyzed. The roots were under two nutritional conditions (only watered or fertilised with 3 mM of ammonium) and harvested after 24 hours of the treatment.

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Transgenic GS1a poplar under different nitrate nutrition level.

Project description:Transcriptional profile of leaves from poplar plants transformed with a pine GS1a gene under two different nitrate nutrition level (50mM and 10mM). Two conditions: nitrate nutrition (50mM and 10 mM) and genotype (GS1a transgenic poplar vs WT poplar)

2015-04-02 | E-GEOD-62449 | biostudies-arrayexpress

Transgenic GS1a poplar under different nitrate nutrition level.

Project description:Transcriptional profile of leaves from poplar plants transformed with a pine GS1a gene under two different nitrate nutrition level (50mM and 10mM).

2015-04-02 | GSE62449 | GEO

Transcriptome analysis of the shoots of Arabidopsis wild-type and ammonium-insensitive mutant plants grown under 10 mM ammonium as sole nitrogen source

Project description:Abundant ammonium nutrition impairs plant growth, i.e. ammonium toxicity, the primary cause of which remains to be determined. To obtain a clue about the toxic mechanism, we performed microarray experiments and compared the expression of genes responsive to toxic levels of ammonium between the wild-type (Col) and an ammonium-insensitive mutant (ami2) shoots growing in media containing 10 mM ammonium.

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