Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data of HNSCC cell lines grown without and with patient-matched CAFs in 3D

ABSTRACT: Expression data of HNSCC cell lines grown without and with patient-matched CAFs in 3D

PROVIDER: PRJNA737483 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Expression data of HNSCC cell lines grown without and with patient-matched CAFs in 3D

Project description:Cancer associated fibroblasts (CAFs) are the major cellular component of the tumor microenvironment and they have been shown to stimulate tumor growth, epithelial-to-mesenchymal transition (EMT), invasion and radio-resistance. We used microarrays to investigate the influence of CAFs on the gene expression profile of HNSCC cells in 3D co-cultures

2021-06-15 | GSE178154 | GEO

Expression data of HNSCC cell lines grown without and with patient-matched CAFs in 2D

2021-06-15 | GSE178153 | GEO

Expression data of HNSCC cell lines grown without and with patient-matched CAFs in 2D

Project description:Expression data of HNSCC cell lines grown without and with patient-matched CAFs in 2D

| PRJNA737482 | ENA

Differentially expressed genes in HNSCC tissues, normal oral epithelial tissues and CAFs

Project description:To identify differentially expressed genes by HNSCC tissues, normal oral epithelial tissues and CAFs were subjected to Agilent whole genome microarrays.

2021-05-01 | GSE172120 | GEO

Differentially expressed micro-RNA in head and neck squamous cell cancer (HNSCC) tissues, normal oral epithelial tissues and CAFs

Project description:Next generation sequencing was perfomed to identify differentially expressed micro-RNA in HNSCC tissues, normal oral epithelial tissues and CAFs.

2021-10-02 | GSE184991 | GEO

scRNA-seq of patient-derived PDAC organoids and matched CAFs

Project description:We established direct three-dimensional (3D) co-cultures of primary PDAC organoids and patient-matched CAFs. Single-cell RNA sequencing was performed for three organoid/CAF pairs in mono- and co-culture to uncover transcriptional changes induced by tumor-stroma interaction. Single-cell RNA sequencing data evidenced induction of a pro-inflammatory phenotype in CAFs in co-cultures. Organoids showed increased expression of genes associated with epithelial-to-mesenchymal transition (EMT) in co-cultures and several potential receptor-ligand interactions related to EMT were identified, supporting a key role of CAF-driven induction of EMT in PDAC chemoresistance.

| EGAS00001006661 | EGA

Gene expression profiling of HNSCC cells derived from primary and matched metastatic tumors

Project description:To look at genes/pathways differentially expressed in metastatic and primary tumor cells we performed global gene expression profiling of the 3 sets of HNSCC lines derived from primary tumors and matched metastatic sites. Illumina HT-12 v4 BeadChip arrays were used. The data suggest that HNSCC lines derived from metastatic sites exhibit phenotypes distinct from those found in cells derived from the corresponding primary tumors. Metastatic cell lines upregulated several pathways involved in stem cell self-renewal, invasion and migration, which are well known characteristics of metastatic progression. We conclude that the cell lines derived from primary patient tumors and matched metastatic sites represent a reliable model to study HNSCC metastasis.

2018-07-27 | GSE117753 | GEO

Next generation sequencing showing miRNA profiles of exosomes derived from DHT-treated CAFs and matched ETOH-treated CAFs

Project description:Background: Androgen deprivation therapy (ADT) is the backbone of therapy for advanced prostate cancer (PCa). Despite the good initial response, castration resistance and metastatic progression will inevitably occur. Cancer-associated fibroblasts (CAFs) may be implicated in promoting metastasis of PCa after ADT. Our aim is to investigate the role and mechanism of CAF-derived exosomes involving in metastasis of PCa after ADT. Methods: PCa cells were co-cultured with exosomes derived from DHT-treated or ETOH-treated CAFs, and their migration and invasion differences under castration condition were examined both in vitro and in vivo. The miRNA profiles of exosomes derived from DHT-treated CAFs and matched ETOH-treated CAFs were analysed via next generation sequencing. The transfer of exosomal miR-146a-5p from CAFs to PCa cells was identified by fluorescent microscopy. The function and direct target gene of exosomal miR-146a-5p in PCa cells were confirmed through Transwell assays, luciferase reporter, and western blot. Findings: Compared with DHT-treated CAFs, exosomes derived from ETOH-treated CAFs dramatically increase migration and invasion of PCa cells under castration condition. MiR-146a-5p level in exosomes from ETOH-treated CAFs was significantly reduced. The loss of miR-146a-5p may strengthen the epithelial-mesenchymal transition (EMT) to accelerate cancer cells metastasis by modulating epidermal growth factor receptor (EGFR)/ERK pathway. Interpretation: CAFs-derived exosomal miR-146a-5p confers metastasis in PCa cells under ADT through the EGFR/ERK pathway and it may present a new treatment for PCa.

2022-08-02 | GSE154215 | GEO

Differentially expressed genes in HNSCC tissues, normal oral epithelial tissues and CAFs

Project description:Differentially expressed genes in HNSCC tissues, normal oral epithelial tissues and CAFs

| PRJNA722177 | ENA

Fibroblasts grown in 3D - Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts

Project description:Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigelTM amenable to stable isotope labeling by amino acids in cell culture and quantitative mass spectrometry-based proteomics analyses. We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAFs) on 3D culture conditions

2018-09-10 | PXD009424 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data