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Transcriptome profiles of Col-0 and scl30a-1 germinating seeds

ABSTRACT: Transcriptome profiles of Col-0 and scl30a-1 germinating seeds

PROVIDER: PRJNA750749 | ENA |

REPOSITORIES: ENA

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Transcriptome profiles of Col-0 and scl30a-1 germinating seeds

Project description:RNA-seq experiment of Col-0 and the scl30a-1 mutant seeds grown for 18 h in continuous white light.

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Project description:The expression profiles were determined using Affymetrix ATH1 arrays. Comparisons among the Col-0, ARF10 and mARF10 sample groups allow the identification of genes regulated by ARF10. Experiment Overall Design: Germinating seeds (22 hr imbibition) from three independent biological replicates of Col-0, ARF10 and mARF10 samples, which contain three independent seed lots each, were analyzed.

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Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the bisulfite sequencing (methylome). Tissue from the msh1 mutant and dcl2,3,4,msh1 quadruple mutants used as rootstocks was similarly collected at the bolting stage and used for the bisulfite sequencing.

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Project description:Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1) and on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on 0.5M MS growth medium and root tissue was collected after 12 days and used for the RNA sequencing.

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Project description:In order to identify differentially expressed genes in developing seeds of Arabidopsis thaliana three different stages of seed development were analysed (9-10, 10-11 and 12-13 days after flower opening) for two Arabidopsis thaliana accessions, Col-0 and C24. For each stage and accession three biological replicates were analysed.

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