MiRNA profiling in heart tissue of AGAT-/- mice
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ABSTRACT:
PROVIDER: PRJNA765950 | ENA |
REPOSITORIES: ENA
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Project description:miRNA sequencing was performed in left ventricular heart tissue of wt (n = 5), AGAT-/- (n = 5), and AGAT-/- mice supplemented with homoarginine (n = 5). Small RNA libraries were prepared using TruSeq Small RNA Sample Preparation Kits (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. The CLC Genomics Workbench (clcbio.com/products/clc-genomics-workbench/) was used to map reads from miRNA sequencing against the murine set of all known miRNAs, which was retrieved from miRBase (www.mirbase.org/). The number of reads falling in mature miRNAs were extracted and further processed in R. Only miRNAs covered by more than ten reads were kept for further analyses. Differential expression of miRNAs between groups of mice was calculated by R/Bioconductor package DESeq2 and the False Discovery Rate (FDR) based Benjamini-Hochberg method was used to account for multiple tests. Differentially expressed miRNAs with an FDR ≤ 0.05 were considered significant.
2022-03-09 | GSE184723 | GEO
Project description:Affymetrix whole genome gene (mRNA) and miRNA expression data during the development period (from E10.5 to E19.5) and expression data of adult (10 weeks old mice) as well as old (14 months old mice) murine heart tissues For a more comprehensive understanding of the potential effects of miRNA for heart development, we carried out the first study of time-resolved parallel profiling of mRNA and miRNA levels in the developing murine heart and identify the dynamical activation or repression of numerous biological processes and signalling pathways
2017-07-31 | GSE93270 | GEO
Project description:As the fetal heart develops, cardiomyocyte proliferation potential decreases while fatty acid oxidative capacity increases, a highly regulated transition known as cardiac maturation. Small noncoding RNAs, such as microRNAs (miRNAs), contribute to the establishment and control of tissue-specific transcriptional programs. However, small RNA expression dynamics and genome wide miRNA regulatory networks controlling maturation of the human fetal heart remain poorly understood. Transcriptome profiling of small RNAs revealed the temporal expression patterns of miRNA, piRNA, circRNA, snoRNA, snRNA and tRNA in the developing human heart between 8 and 19 weeks of gestation. Our analysis revealed that miRNAs were the most dynamically expressed small RNA species throughout mid-gestation. Cross-referencing differentially expressed miRNAs and mRNAs predicted 6,200 mRNA targets, 2134 of which were upregulated and 4066 downregulated as gestation progresses. Moreover, we found that downregulated targets of upregulated miRNAs predominantly control cell cycle progression, while upregulated targets of downregulated miRNAs are linked to energy sensing and oxidative metabolism. Furthermore, integration of miRNA and mRNA profiles with proteomes and reporter metabolites revealed that proteins encoded in mRNA targets, and their associated metabolites, mediate fatty acid oxidation and are enriched as the heart develops.This study revealed the small RNAome of the maturing human fetal heart. Furthermore, our findings suggest that coordinated activation and repression of miRNA expression throughout mid-gestation is essential to establish a dynamic miRNA-mRNA-protein network that decreases cardiomyocyte proliferation potential while increasing the oxidative capacity of the maturing human fetal heart.
2024-01-26 | PXD044936 | Pride
Project description:baylor vaccine project, mice heart tissue RA, LC MS run data, 03 17 2021
2021-03-26 | MSV000087112 | MassIVE
Project description:Baylor vaccine project run start at 3 17 2021, mice heart tissue LA section
2021-03-26 | MSV000087111 | MassIVE
Project description:miRNA profiling of MWCNT exposed mice
| PRJNA542675 | ENA
Project description:Purpose: Identification of miRNA expression profiles of selected horse tissues. Methods: miRNA-seq analysis was performed on lung, heart and liver samples collected from 4 horses. The miRNA libraries were constructed from total RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer protocol. The quantification of the obtained libraries was performed on a Qubit 2.0 spectrophotometer (Invitrogen, Life Technologies), while a quality control on a TapeStation 2200 instrument (D1000 ScreenTape; Agilent). 75 single-end cycle sequencing was performed on the NextSeq 500/550 platform (Illumina) with the use of NextSeq High Output Reagents v2 (Illumina). Results: The comparison of miRNA profiles between the investigated tissues revealed 185 differentially expressed microRNAs (p adj≤0.05) in the heart samples versus the liver samples, 172 DE miRNAs (p adj ≤0.05) in the lung samples in comparison to the liver samples, and 155 in the lung samples versus the heart samples. Conclusions: Obtained results show varied miRNA expression profiles characteristics for each investigated hore tissue.
2023-06-01 | GSE202502 | GEO
Project description:Transcriptomic study of heart tissue from diabetic cardiomyopathy mice
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Project description:Mutations in the gene for the mitochondrial matrix protease CLPP can cause human Perrault syndrome, which is characterized by male and female infertility, progressive sensorineural deafness, ataxia and leukoencephalopathy. This gene encodes a peptidase that is conserved since bacteria and localizes to mitochondrial matrix in eukaryotes. To assess the assembly and stability of mitochondrial complexes heart tissue of WT and ClpP-/- mice was dissected and enriched mitochondrial fraction was used for complexome profiling.
2024-01-17 | PXD036933 | Pride
Project description:UQCRH, also called the hinge protein, is a small subunit of Ubiquinol—cytochrome-c reductase (complex III). Here we used a complexome analysis of heart tissue of UQCRH deficient mice to study complex III assembly and altered supercomplex composition.
2021-09-20 | PXD022855 | Pride